利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

27 条记录 (耗时 0.03 秒)

    METHODS OF DETERMINING POTENCY OF CHEMICALLY-SYNTHESIZED OLIGONUCLEOTIDES
    3.
    发明申请
    METHODS OF DETERMINING POTENCY OF CHEMICALLY-SYNTHESIZED OLIGONUCLEOTIDES 有权
    确定化学合成寡核苷酸的方法

    公开(公告)号：US20100267149A1

    公开(公告)日：2010-10-21

    申请号：US12445854

    申请日：2007-10-18

    申请人： Walter F. Lima ,  Timothy Vickers ,  Stanley T. Crooke

    发明人： Walter F. Lima ,  Timothy Vickers ,  Stanley T. Crooke

    IPC分类号： G01N33/53

    CPC分类号： C12Q1/6876 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2320/10 ,  Y10T436/143333

    摘要： Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.

    摘要翻译： 本文提供了确定RNAi剂效力的方法。 这样的方法包括但不限于测量RNAi剂与Ago2的结合或在这种RNAi剂存在下测量Ago2活性的基于细胞和无细胞的测定。 还提供了通过评估其与其他RNAi试剂（包括对照RNAi试剂）竞争与Ago2结合和/或激活的能力来测定RNAi试剂的效力的测定法。

    EFFICIENT REDUCTION OF TARGET RNA'S BY SINGLE- AND DOUBLE-STRANDED OLIGOMERIC COMPOUNDS
    4.
    发明申请
    EFFICIENT REDUCTION OF TARGET RNA'S BY SINGLE- AND DOUBLE-STRANDED OLIGOMERIC COMPOUNDS 审中-公开
    单链和双链寡聚化合物有效减少目标RNA

    公开(公告)号：US20100041047A1

    公开(公告)日：2010-02-18

    申请号：US12511437

    申请日：2009-07-29

    申请人： Timothy Vickers ,  Seongjoon Koo ,  C. Frank Bennett ,  Stanley T. Crooke ,  Nicholas M. Dean ,  Brenda F. Baker

    发明人： Timothy Vickers ,  Seongjoon Koo ,  C. Frank Bennett ,  Stanley T. Crooke ,  Nicholas M. Dean ,  Brenda F. Baker

    IPC分类号： C12Q1/68 ,  C07H21/02

    CPC分类号： C12N15/1138 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2310/53 ,  C12N2310/3525

    摘要： The present invention provides, inter alia, methods of selecting a single-stranded oligomeric compounds for inhibiting RNA expression, methods of generating double-stranded oligomeric compounds, methods of identifying optimized double-stranded oligomeric compounds, methods of selecting optimized single-stranded oligomeric compounds, methods of selecting optimized double-stranded oligomeric compounds, methods of identifying multifunctional oligomeric compounds, methods for optimizing target region selection for modulation of RNA expression, methods of optimizing expression modulation of RNA, and the like. The present invention further provides oligomeric compounds, 8-80 nucleobases in length targeted to a target RNA, wherein said oligomeric compound hybridizes to said target RNA and inhibits RNA levels by at least 50% in both single-stranded and double-stranded forms, and multifunctional oligomeric compounds.

    摘要翻译： 本发明尤其提供了选择用于抑制RNA表达的单链寡聚化合物的方法，产生双链寡聚化合物的方法，鉴定优化的双链寡聚化合物的方法，选择优化的单链寡聚化合物的方法 选择优化的双链寡聚化合物的方法，鉴定多功能寡聚化合物的方法，用于优化用于调节RNA表达的靶区选择的方法，优化RNA表达调节的方法等。 本发明进一步提供低聚化合物，长度靶向靶RNA的8-80个核碱基，其中所述寡聚化合物与所述靶RNA杂交并且以单链和双链形式抑制至少50％的RNA水平，以及 多官能寡聚化合物。

    METHODS OF DETERMINING POTENCY OF CHEMICALLY-SYNTHESIZED OLIGONUCLEOTIDES
    9.
    发明申请
    METHODS OF DETERMINING POTENCY OF CHEMICALLY-SYNTHESIZED OLIGONUCLEOTIDES 有权
    确定化学合成寡核苷酸的方法

    公开(公告)号：US20120077276A1

    公开(公告)日：2012-03-29

    申请号：US13311366

    申请日：2011-12-05

    申请人： Walter F. Lima ,  Timothy Vickers ,  Stanley T. Crooke

    发明人： Walter F. Lima ,  Timothy Vickers ,  Stanley T. Crooke

    IPC分类号： G01N33/50

    CPC分类号： C12Q1/6876 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2320/10 ,  Y10T436/143333

    摘要： Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.

    摘要翻译： 本文提供了确定RNAi剂效力的方法。 这样的方法包括但不限于测量RNAi剂与Ago2的结合或在这种RNAi剂存在下测量Ago2活性的基于细胞和无细胞的测定。 还提供了通过评估其与其他RNAi试剂（包括对照RNAi试剂）竞争与Ago2结合和/或激活的能力来测定RNAi试剂的效力的测定法。