公开(公告)号：US20230125093A1

公开(公告)日：2023-04-27

申请号：US17970874

申请日：2022-10-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Kevin Wyndham ,  Szabolcs Fekete ,  Hua Yang ,  Stephan Koza

IPC: C12N15/10

Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.