公开(公告)号：US20210322896A1

公开(公告)日：2021-10-21

申请号：US17231808

申请日：2021-04-15

Applicant: Waters Technologies Corporation

Inventor： Pamela C. Iraneta ,  Stephen James Shiner ,  Raymond P. Fisk ,  Lavelay Kizekai

IPC: B01D15/34 ,  B01D71/06 ,  B01J20/281

Abstract: This disclosure pertains to performance enhancing conditioning and storage solvents containing low levels of buffer and salt for reproducible and improved size exclusion chromatography (SEC). In some embodiments, the present disclosure pertains to storage solvents for protein-based size exclusion chromatography that comprise water, a water-miscible organic solvent, a phosphate salt, and a neutral salt. In some embodiments, the present disclosure pertains to columns for protein-based size exclusion chromatography that comprise porous particles and such a storage solvent

公开(公告)号：US11951417B2

公开(公告)日：2024-04-09

申请号：US17231808

申请日：2021-04-15

Applicant: Waters Technologies Corporation

Inventor： Pamela C. Iraneta ,  Stephen James Shiner ,  Raymond P. Fisk ,  Lavelay Kizekai

IPC: B01D15/20 ,  B01D15/34 ,  B01D71/06 ,  G01N30/50

CPC classification number: B01D15/20 ,  B01D15/34 ,  B01D71/06 ,  G01N30/50

Abstract: This disclosure pertains to performance enhancing conditioning and storage solvents containing low levels of buffer and salt for reproducible and improved size exclusion chromatography (SEC). In some embodiments, the present disclosure pertains to storage solvents for protein-based size exclusion chromatography that include water, a water-miscible organic solvent, a phosphate salt, and a neutral salt. In some embodiments, the present disclosure pertains to columns for protein-based size exclusion chromatography that include porous particles and such a storage solvent.

公开(公告)号：US20210325352A1

公开(公告)日：2021-10-21

申请号：US17231853

申请日：2021-04-15

Applicant: Waters Technologies Corporation

Inventor： Stephen James Shiner ,  Frank J. Marszalkowski, Jr. ,  Steven M. Byrd ,  Raymond P. Fisk ,  Mathew H. DeLano ,  Lavelay Kizekai

IPC: G01N30/60

Abstract: Chromatographic systems for size exclusion chromatography (SEC) are provided that comprise an inlet, an outlet, an analytic column having a first interior volume that has a first length and a first cross-sectional area normal to the first length, the first interior volume containing a first stationary phase, and a guard column having a second interior volume that has a second length and a second cross-sectional area normal to the second length, the second interior volume containing a second stationary phase. The inlet is in fluid communication with the guard column, the guard column is in fluid communication with the analytic column, and the analytic column is in fluid communication with the outlet. Moreover, the second length is smaller than the first length, and the second cross-sectional area is smaller than the first cross-sectional area.