公开(公告)号：US4987539A

公开(公告)日：1991-01-22

申请号：US082254

申请日：1987-08-05

申请人： Wayne A. Moore ,  Tom Nozaki, Jr. ,  David R. Parks ,  Richard T. Stovel ,  Gary Breitbard

发明人： Wayne A. Moore ,  Tom Nozaki, Jr. ,  David R. Parks ,  Richard T. Stovel ,  Gary Breitbard

IPC分类号： B07C5/342 ,  G01N15/14

CPC分类号： B07C5/3427 ,  G01N15/14 ,  G01N2015/149

摘要： A system for characterizing objects to be sorted in real time and making sorting decisions using lasers to excite fluorescing dyes used to tag objects such as cells which flow through the laser beam in a stream of conductive fluid. The scattered light and fluorescent light at various frequencies define a multidimensional space. These light characteristics are sensed and digitized. The data so gathered is buffered and then compared to a binary classification tree. The individual parameters cause a certain path through the binary decision tree to be taken while the cell is passing from the point in the stream where the light characteristics are gathered to a point where electrostatically charged electrodes deflect individual statically charged drops containing cells to be sorted into various sorting bins. When the decision tree has been traversed, the sorting decision for the drop containing the cell which had those parameters is done. This sorting decision is buffered until the time the drop reaches the charged electrodes. The sort decision is then accessed and used to properly charge the electrodes so that the drop is diverted into the proper bin.

摘要翻译： 用于表征要实时分类的对象的系统，并且使用激光器进行排序决定，以激发用于标记诸如在导电流体流中流过激光束的细胞的物体的荧光染料。 各种频率的散射光和荧光灯定义了多维空间。 这些光特性被感测和数字化。 如此收集的数据被缓冲，然后与二进制分类树进行比较。 单个参数导致通过二进制决策树的特定路径，同时单元从光聚合的流中的点通过到静电充电电极偏转包含待分类的单元的静态电荷的液滴的点 各种分拣箱。 当决策树已经遍历时，对包含具有这些参数的单元的分支的排序决定完成。 这种排序决定被缓冲，直到液滴到达带电电极。 然后访问排序决定并将其用于适当地对电极充电，使得液滴转入适当的料仓。