公开(公告)号：US4667830A

公开(公告)日：1987-05-26

申请号：US273750

申请日：1981-06-15

申请人： Tom Nozaki, Jr. ,  Richard G. Sweet ,  Richard T. Stovel ,  David R. Parks

发明人： Tom Nozaki, Jr. ,  Richard G. Sweet ,  Richard T. Stovel ,  David R. Parks

IPC分类号： B07C5/342 ,  G01N15/14

CPC分类号： B07C5/3427 ,  G01N2015/149

摘要： Method and apparatus for sorting individual particles into containers, such as the wells of a microculture tray, are disclosed. The particles may comprise, for example, individual biological cells which then may be cultured, cloned, analyzed, or the like. The particles are incorporated into a flow stream which is jetted from a nozzle which is vibrated in the direction of the stream to break the jet stream into discrete drops. A particle sensor senses particles in the stream, and a signal processor in response to the particle sensor output produces an output when the sensed particle is of the type to be sorted from the stream. A pulse charger, responsive to the signal processor, is included for charging the drops as they break from the vibrating jet stream. The drops pass between charged deflection plates where they are deflected an amount related to the drop charge. For individual cell sorting, the signal processor is in normal inopertive condition to prevent sorting of drops from the drop stream. During this time all drops contained in the stream are caught by a stream catcher, and none are allowed to enter a tray well. When armed, by manual actuation of an arming switch, the circuit is conditioned to sort a single particle from the drop stream, at which time the circuit returns to normal inoperative condition. A tray support is provided for movable support of the multiwell tray along perpendicular X and Y directions, and a releasable detent releasably lock the tray support at any desired X-Y position. The tray support is provided with a target for use in initial alignment of the tray support relative to drops separated from the stream to assure entry of such drops into a tray well during subsequent operation of the cell sorter.

摘要翻译： 公开了将各种颗粒分离成容器的方法和装置，例如微型培养皿的孔。 颗粒可以包括例如可以培养，克隆，分析等的单个生物细胞。 将颗粒结合到从喷嘴喷射的流中，该喷嘴在流动方向上振动以将喷射流破裂成离散的液滴。 粒子传感器感测流中的粒子，并且响应于粒子传感器输出的信号处理器当感测到的粒子是从流中排序的类型时产生输出。 包括响应于信号处理器的脉冲充电器，用于在液滴从振动喷射流中分离时对其充电。 液滴在带电偏转板之间通过，在它们之间偏转与滴落电荷相关的量。 对于单个细胞分选，信号处理器处于正常的无效状态，以防止液滴从滴流中分选。 在此期间，流中包含的所有液滴都被流捕获器捕获，并且不允许任何进入盘。 当装备时，通过手动启动布防开关，电路被调节为从液滴流中分离单个颗粒，此时电路恢复到正常的不工作状态。 提供了托盘支撑件，用于沿着垂直X和Y方向的多孔托盘的可移动支撑件，以及可释放的止动件将托盘支撑件可释放地锁定在任何期望的X-Y位置。 托盘支撑件设置有用于托盘支撑件相对于从流分离的液滴的初始对准中的目标，以确保在细胞分选器的后续操作期间将这些液滴进入托盘孔。

公开(公告)号：US4987539A

公开(公告)日：1991-01-22

申请号：US082254

申请日：1987-08-05

申请人： Wayne A. Moore ,  Tom Nozaki, Jr. ,  David R. Parks ,  Richard T. Stovel ,  Gary Breitbard

发明人： Wayne A. Moore ,  Tom Nozaki, Jr. ,  David R. Parks ,  Richard T. Stovel ,  Gary Breitbard

IPC分类号： B07C5/342 ,  G01N15/14

CPC分类号： B07C5/3427 ,  G01N15/14 ,  G01N2015/149

摘要： A system for characterizing objects to be sorted in real time and making sorting decisions using lasers to excite fluorescing dyes used to tag objects such as cells which flow through the laser beam in a stream of conductive fluid. The scattered light and fluorescent light at various frequencies define a multidimensional space. These light characteristics are sensed and digitized. The data so gathered is buffered and then compared to a binary classification tree. The individual parameters cause a certain path through the binary decision tree to be taken while the cell is passing from the point in the stream where the light characteristics are gathered to a point where electrostatically charged electrodes deflect individual statically charged drops containing cells to be sorted into various sorting bins. When the decision tree has been traversed, the sorting decision for the drop containing the cell which had those parameters is done. This sorting decision is buffered until the time the drop reaches the charged electrodes. The sort decision is then accessed and used to properly charge the electrodes so that the drop is diverted into the proper bin.

摘要翻译： 用于表征要实时分类的对象的系统，并且使用激光器进行排序决定，以激发用于标记诸如在导电流体流中流过激光束的细胞的物体的荧光染料。 各种频率的散射光和荧光灯定义了多维空间。 这些光特性被感测和数字化。 如此收集的数据被缓冲，然后与二进制分类树进行比较。 单个参数导致通过二进制决策树的特定路径，同时单元从光聚合的流中的点通过到静电充电电极偏转包含待分类的单元的静态电荷的液滴的点 各种分拣箱。 当决策树已经遍历时，对包含具有这些参数的单元的分支的排序决定完成。 这种排序决定被缓冲，直到液滴到达带电电极。 然后访问排序决定并将其用于适当地对电极充电，使得液滴转入适当的料仓。

公开(公告)号：US4367043A

公开(公告)日：1983-01-04

申请号：US146219

申请日：1980-05-05

申请人： Richard G. Sweet ,  Wayne A. Moore ,  Tom Nozaki, Jr. ,  Richard T. Stovel

发明人： Richard G. Sweet ,  Wayne A. Moore ,  Tom Nozaki, Jr. ,  Richard T. Stovel

IPC分类号： G01N1/00 ,  G01N21/11 ,  G01N15/02 ,  G01N21/00

CPC分类号： G01N35/1097

摘要： Method and apparatus for semi-automatic feeding of liquid samples from a plurality of sample sources to a scanning device are disclosed. The liquid samples are picked up from a preidentified sample source and delivered to the scanning device by use of a hand-held pick-up and delivery tube. The hand-held tube is releasably coupled to the scanning device for delivery of the liquid samples to the scanning device when coupled thereto. In the disconnected condition, particle free fluid flows in a reverse direction from the scanning device for automatic flushing of the scanning device and liquid sample supply line connected thereto. Visual display means for visually indicating to the operator which sample holder the next sample is to be obtained from is provided.

摘要翻译： 公开了用于将多个样品源的液体样品半自动进给到扫描装置的方法和装置。 液体样品从预先确定的样品源中取出，并通过使用手提式拾取和输送管道输送到扫描装置。 手持管可释放地联接到扫描装置，用于当与其连接时将液体样品传送到扫描装置。 在断开状态下，无颗粒流体从扫描装置反向流动，用于自动冲洗与其连接的扫描装置和液体样品供应管线。 视觉显示装置用于向操作者可视地指示要获得下一个样品的样品架。