公开(公告)号：US20060281147A1

公开(公告)日：2006-12-14

申请号：US10595576

申请日：2003-10-31

申请人： Weon Kyoo You ,  Won Seok Choi ,  You Seok Koh ,  Seung Woo Kang ,  Kwang Hoe Chung

发明人： Weon Kyoo You ,  Won Seok Choi ,  You Seok Koh ,  Seung Woo Kang ,  Kwang Hoe Chung

IPC分类号： C12P21/06 ,  C12N9/74 ,  C07H21/04 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： A recombinant thrombin-like enzyme batroxobin expressed by yeast, a production method thereof, and use of the batroxobin as a hemostatic agent or antithrombotic agent is disclosed. In order to express recombinant thrombin-like enzyme, an expression vector is prepared by inserting cDNA encoding the enzyme, the transformed cell is prepared by introducing the expression vector into the cell, the transformed cell then is incubated and the recombinant thrombin-like enzyme is obtained from the cell. The recombinant thrombin-like enzyme may be usefully used as a hemostatic agent because the enzyme effectively decrease bleeding time and blood coagulation time and does not affect various blood coagulation factors. The recombinant thrombin-like enzyme is also useful for a hemostatic agent or antithrombotic agent comprising the recombinant thrombin-like enzyme as an active component

摘要翻译： 公开了由酵母表达的重组凝血酶样酶巴曲酶，其制备方法，以及巴曲酶作为止血剂或抗血栓剂的用途。 为了表达重组凝血酶样酶，通过插入编码该酶的cDNA来制备表达载体，通过将表达载体导入细胞来制备转化细胞，然后将转化的细胞孵育，重组凝血酶样酶为 从细胞获得。 重组凝血酶样酶可有效地用作止血剂，因为该酶有效地减少了出血时间和凝血时间，并且不影响各种凝血因子。 重组凝血酶样酶还可用于包含重组凝血酶样酶作为活性成分的止血剂或抗血栓形成剂