公开(公告)号：US20250108131A1

公开(公告)日：2025-04-03

申请号：US18899814

申请日：2024-09-27

Applicant: Whitehead Institute for Biomedical Research

Inventor： Jonathan Weissman ,  Mohamed El-Brolosy ,  André Fischer ,  Atharv Vivek Oak

IPC: A61K48/00 ,  A61K38/17 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6804 ,  C12Q1/6869 ,  G01N33/50

Abstract: The present disclosure, at least in part, relates to compositions (e.g., engineered nucleic acids and engineered proteins) and methods for increasing gene expression. The engineered proteins include RNA-binding proteins (e.g., RNA-binding proteins that comprise a Interleukin Enhancer Binding Factor 3 (ILF3) sequence, a Cas sequence, or a combination thereof). In some aspects, the disclosure provides methods of identifying engineered nucleic acids that are shorter in length than a gene of interest to induce expression of the gene of interest and also provides RNA-binding proteins for inducing gene expression.