公开(公告)号：US20250108131A1

公开(公告)日：2025-04-03

申请号：US18899814

申请日：2024-09-27

Applicant: Whitehead Institute for Biomedical Research

Inventor： Jonathan Weissman ,  Mohamed El-Brolosy ,  André Fischer ,  Atharv Vivek Oak

IPC: A61K48/00 ,  A61K38/17 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6804 ,  C12Q1/6869 ,  G01N33/50

Abstract: The present disclosure, at least in part, relates to compositions (e.g., engineered nucleic acids and engineered proteins) and methods for increasing gene expression. The engineered proteins include RNA-binding proteins (e.g., RNA-binding proteins that comprise a Interleukin Enhancer Binding Factor 3 (ILF3) sequence, a Cas sequence, or a combination thereof). In some aspects, the disclosure provides methods of identifying engineered nucleic acids that are shorter in length than a gene of interest to induce expression of the gene of interest and also provides RNA-binding proteins for inducing gene expression.

公开(公告)号：US12215361B2

公开(公告)日：2025-02-04

申请号：US18444590

申请日：2024-02-16

Applicant: Whitehead Institute for Biomedical Research

Inventor： Edwin Neumann ,  Tessa Bertozzi ,  Jonathan Weissman

IPC: C12N9/10 ,  A61K38/46 ,  C12N9/22 ,  C12N15/86

Abstract: The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.

公开(公告)号：US20250027059A1

公开(公告)日：2025-01-23

申请号：US18894575

申请日：2024-09-24

Applicant: Whitehead Institute for Biomedical Research

Inventor： Edwin Neumann ,  Tessa Bertozzi ,  Jonathan Weissman

IPC: C12N9/10 ,  A61K38/46 ,  C12N9/22 ,  C12N15/86

Abstract: The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.

公开(公告)号：US12252715B2

公开(公告)日：2025-03-18

申请号：US18444590

申请日：2024-02-16

Applicant: Whitehead Institute for Biomedical Research

Inventor： Edwin Neumann ,  Tessa Bertozzi ,  Jonathan Weissman

IPC: C12N9/10 ,  A61K38/46 ,  C12N9/22 ,  C12N15/86

Abstract: The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.

公开(公告)号：US20250019673A1

公开(公告)日：2025-01-16

申请号：US18894709

申请日：2024-09-24

Applicant: Whitehead Institute for Biomedical Research

Inventor： Edwin Neumann ,  Tessa Bertozzi ,  Jonathan Weissman

IPC: C12N9/10 ,  A61K38/46 ,  C12N9/22 ,  C12N15/86

Abstract: The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.

公开(公告)号：US20240279623A1

公开(公告)日：2024-08-22

申请号：US18444590

申请日：2024-02-16

Applicant: Whitehead Institute for Biomedical Research

Inventor： Edwin Neumann ,  Tessa Bertozzi ,  Jonathan Weissman

IPC: C12N9/10 ,  A61K38/46 ,  C12N9/22 ,  C12N15/86

CPC classification number: C12N9/1007 ,  A61K38/465 ,  C12N9/22 ,  C12N15/86 ,  C12Y201/01037 ,  C07K2319/80 ,  C12N2750/14143

Abstract: The disclosure provides, in various embodiments, fusion proteins comprising a DNA-binding domain, a DNMT3A-binding domain, and a H3K4me0; and polynucleotides and vectors encoding one or more of the fusion proteins. The disclosure also provides, in various embodiments, gene-delivery systems, cells, compositions (e.g., pharmaceutical compositions) and kits comprising one or more of the fusion proteins polynucleotides, or vectors; methods of epigenetically modifying a genomic locus in a cell; and methods of treating a subject (e.g., a human) in need thereof.