公开(公告)号：US09304307B2

公开(公告)日：2016-04-05

申请号：US13902763

申请日：2013-05-24

申请人： Wibke Hellmich ,  Gerhard Krampert ,  Matthias Langhorst ,  Ralf Netz

发明人： Wibke Hellmich ,  Gerhard Krampert ,  Matthias Langhorst ,  Ralf Netz

IPC分类号： G02B26/02 ,  G02B21/00

CPC分类号： G02B21/0044 ,  G02B21/0052 ,  G02B21/008

摘要： A light scanning microscope with an illumination module generates several illumination beams and moves them, in each case as a spot, in a predefined region of a sample to excite sample radiation. A detector module for confocal detection of the sample radiation excited by each spot includes a first detector, an imaging lens system, having an optical axis, for imaging the predefined region along an imaging beam path running from the sample as far as the first detector, and a rotatable diaphragm with several pinholes located in a pinhole plane. The diaphragm, upon rotation, may be located at least partially in the imaging beam path for confocal detection. A second detector may be arranged outside of the imaging beam path. A first beam splitter may be arranged in the imaging beam path between the sample and the diaphragm. The beam splitter deflects sample radiation onto the second detector.

摘要翻译： 具有照明模块的光扫描显微镜产生几个照明光束，并将它们在每种情况下作为点移动到样品的预定区域以激发样品辐射。 用于由每个点激发的样品辐射的共焦检测的检测器模块包括具有光轴的第一检测器，成像透镜系统，用于沿着从样品运行的成像光束路径成像到第一检测器， 以及具有位于针孔平面中的几个针孔的可旋转隔膜。 膜片在旋转时可以至少部分地位于用于共焦检测的成像光束路径中。 第二检测器可以布置在成像光束路径的外部。 第一分束器可以布置在样品和隔膜之间的成像光束路径中。 分束器将样品辐射器偏转到第二检测器上。

公开(公告)号：US20110094318A1

公开(公告)日：2011-04-28

申请号：US12997113

申请日：2009-06-06

申请人： Wibke Hellmich ,  Benno Radt ,  Helmut Lippert ,  Olaf Selchow ,  Jürgen Haese ,  Uwe Wolf

发明人： Wibke Hellmich ,  Benno Radt ,  Helmut Lippert ,  Olaf Selchow ,  Jürgen Haese ,  Uwe Wolf

IPC分类号： G01N1/28

CPC分类号： G01N1/28 ,  G02B21/32 ,  G02B21/34

摘要： The invention is directed to method for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement. According to the invention, the method mentioned above has the following method steps: a sample is introduced into a transparent medium, preferably agarose gel, which is initially liquid; the medium is changed from the liquid state to the solid state, wherein the sample is fixated within the medium, but the transparency of the medium is retained; the solidified medium is positioned in the microscope arrangement in such a way that the sample enclosed therein is situated in the detection area of the objective. Further, a device is proposed for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement.

摘要翻译： 本发明涉及在显微镜装置的目标的检测区域中定位和对准优选生物样品的方法。 根据本发明，上述方法具有以下方法步骤：将样品引入最初为液体的透明介质，优选琼脂糖凝胶; 介质从液态变为固态，其中样品固定在介质中，但保持介质的透明度; 固化介质以这样一种方式定位在显微镜装置中，使得封闭在其中的样品位于物镜的检测区域中。 此外，提出了一种用于在显微镜装置的目标的检测区域中定位和对准优选生物样品的装置。