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公开(公告)号：US5272058A
公开(公告)日：1993-12-21
申请号：US479691
申请日：1990-02-13
IPC分类号： A61K39/00 , C07K14/44 , C07K16/20 , C12Q1/68 , G01N33/569 , C12Q1/00 , G01N33/53 , G01N33/566 , A61K35/14 , C07K3/00 , C07K13/00 , C07K15/00 , C07K17/00
CPC分类号： C07K16/20 , C07K14/44 , C12Q1/6893 , G01N33/56905 , A61K39/00 , Y10S435/961 , Y10S435/975
摘要： A simple immunoassay method is provided which distinguishes pathogenic from nonpathogenic forms Entamoeba histolytica in biological samples. This assay utilizes monoclonal antibody preparations which are specific for designated epitopes of the 170 kd subunit of the Gal/GalNAc lectin. When pathogenic forms, specifically, are to be detected, at least one antibody which is immunospecific for an epitope unique to the forms of the 170 kd lectin found in pathogenic strains is used in the assay. The invention further includes monoclonal antibodies which are immunospecific for epitopes 1-3 of the 170 kd subunit of the Gal/GalNAc lectin of either pathogenic or nonpathogenic forms and to monoclonal antibodies specifically immunoreactive with epitopes unique to nonpathogenic derived 170 kd subunit, as well as purified forms of the Gal/GalNAc lectin from both pathogenic and nonpathogenic forms.
摘要翻译： 提供了简单的免疫测定方法，其在生物样品中区分致病与非病原体形式的溶组织内阿米巴。 该测定使用对Gal / GalNAc凝集素的170kd亚基的指定表位特异的单克隆抗体制剂。 当要检测到特异性的致病形式时，在测定中使用至少一种针对在致病菌株中发现的170kd凝集素形式特有的表位具有免疫特异性的抗体。 本发明还包括针对致病性或非致病性形式的Gal / GalNAc凝集素的170kd亚基的表位1-3具有免疫特异性的单克隆抗体以及与非致病性衍生的170kd亚基特有的表位特异性免疫反应的单克隆抗体，以及 Gal / GalNAc凝集素的纯化形式来源于致病和非病原体形式。
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2.发明授权Monoclonal antibodies for differentiating pathogenic and nonpathogenic E. histolytica 失效用于分化病原性和非致病性E.组织溶液的单克隆抗体
公开(公告)号：US5401831A
公开(公告)日：1995-03-28
申请号：US75226
申请日：1993-06-10
IPC分类号： C07K14/44 , C07K16/20 , C12Q1/68 , G01N33/569 , C07K15/28
CPC分类号： G01N33/56905 , C07K14/44 , C07K16/20 , C12Q1/6893 , Y10S530/822
摘要： A method to prepare a monoclonal antibody (mAb) specifically immunoreactive with an epitope of the Gal/GalNAc lectin of a pathogenic or nonpathogenic E. histolytica, or the 70 kd subunit thereof, is provided. This method comprises culturing an immortalized cell line capable of secreting the mAbs, where the cell line is obtained by immortalizing antibody-producing cells from a mammal immunized with a purified 170 kd subunit, or with purified Gal/GalNAc lectin of a pathogenic E. histolytica. The supernatants of the cells are screened for the presence or absence of antibodies specific for the subunit or lectin from a pathogenic E. histolytica and from a pathogenic E. histolytica, so as to determine the pathogen/nonpathogen specificity of the monoclonal antibody. Antibodies produced by this method and epitopes with which they react on pathogenic and/or nonpathogenic E. histolytica also are disclosed.
摘要翻译： 提供了与致病性或非致病性E.组织酵解的Gal / GalNAc凝集素的表位特异性免疫反应的单克隆抗体（mAb）或其70kd亚基的方法。 该方法包括培养能够分泌mAb的永生化细胞系，其中细胞系通过使用纯化的170kd亚单位免疫的哺乳动物产生抗体的细胞永生化，或用致病性E.组织解毒素的纯化Gal / GalNAc凝集素 。 筛选细胞的上清液，以存在或不存在来自病原性组织解毒液的亚单位或凝集素特异性抗体和来自病原性组织溶组织酵母，以确定单克隆抗体的病原体/非病原体特异性。 还公开了通过该方法产生的抗体和与病原性和/或非致病性E.组织溶液反应的表位。
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公开(公告)号：US5405748A
公开(公告)日：1995-04-11
申请号：US75222
申请日：1993-06-10
CPC分类号： C07K16/20 , C07K14/44 , C12Q1/6893 , G01N33/56905 , A61K39/00 , Y10S435/961 , Y10S435/975
摘要： A simple immunoassay method is provided which distinguishes pathogenic from nonpathogenic forms Entamoeba histolytica in biological samples. This assay utilizes monoclonal antibody preparations which are specific for designated epitopes of the 170 kd subunit of the Gal/GalNAc lectin. When pathogenic forms, specifically, are to be detected, at least one antibody which is immunospecific for an epitope unique to the forms of the 170 kd lectin found in pathogenic strains is used in the assay. The invention further includes monoclonal antibodies which are immunospecific for epitopes 1-3 of the 170 kd subunit of the Gal/GalNAc lectin of either pathogenic or nonpathogenic forms and to monoclonal antibodies specifically immunoreactive with epitopes unique to nonpathogenic derived 170 kd subunit, as well as purified forms of the Gal/GalNAc lectin from both pathogenic and nonpathogenic forms.
摘要翻译： 提供了简单的免疫测定方法，其在生物样品中区分致病与非病原体形式的溶组织内阿米巴。 该测定使用对Gal / GalNAc凝集素的170kd亚基的指定表位特异的单克隆抗体制剂。 当要检测到特异性的致病形式时，在测定中使用至少一种针对在致病菌株中发现的170kd凝集素形式特有的表位具有免疫特异性的抗体。 本发明还包括针对致病性或非致病性形式的Gal / GalNAc凝集素的170kd亚基的表位1-3具有免疫特异性的单克隆抗体以及与非致病性衍生的170kd亚基特有的表位特异性免疫反应的单克隆抗体，以及 Gal / GalNAc凝集素的纯化形式来源于致病和非病原体形式。
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公开(公告)号：US5004608A
公开(公告)日：1991-04-02
申请号：US456579
申请日：1989-12-29
摘要： Purified Gal/GalNAc adherence lectin of Entamoeba histolytica is used for development of a vaccine to prevent human amebiasis.
摘要翻译： 溶组织内阿米巴的纯化Gal / GalNAc粘附凝集素被用于开发用于预防人类阿米巴病的疫苗。
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