公开(公告)号：US20130344596A1

公开(公告)日：2013-12-26

申请号：US13982766

申请日：2012-02-03

申请人： William E. Grieshober, JR. ,  James S. Jones ,  Semyon Kogan ,  Ilya Ilyin ,  Natella I. Enukashvily ,  Yana A. Filkina ,  Alexander N. Shumeev ,  Stanislav Kolchanov

发明人： William E. Grieshober, JR. ,  James S. Jones ,  Semyon Kogan ,  Ilya Ilyin ,  Natella I. Enukashvily ,  Yana A. Filkina ,  Alexander N. Shumeev ,  Stanislav Kolchanov

IPC分类号： A01N1/02

CPC分类号： A01N1/0284 ,  A01N1/0289

摘要： Provided is a method for reducing apoptosis in nucleated cells. The method entails holding nucleated cells in a container and adding a gas containing xenon to the container so that the pressure inside the container reaches between 0.5 to 4.0 Atm above ambient pressure; holding the container at between 0.5 to 4.0 Atm above ambient pressure for a period of time during which the temperature in the container is between 22° C. and 37° C.; lowering the temperature in the container to between 0.1° C. and 10° C. while maintaining the pressure of 0.5 to 4.0 Atm above ambient pressure and holding the container for a period of time; and reducing the pressure in the container to ambient pressure and increasing the temperature to 22° C.-37° C. By performing these steps, the cells undergo less apoptosis than a reference.

摘要翻译： 提供了一种减少有核细胞凋亡的方法。 该方法需要将有核细胞保持在容器中，并将含有氙气体添加到容器中，使得容器内的压力达到高于环境压力的0.5至4.0atm; 将容器保持在高于环境压力的0.5至4.0atm之间一段时间，在此期间容器中的温度在22℃至37℃之间; 将容器中的温度降至0.1℃至10℃之间，同时保持高于环境压力的0.5至4.0atm的压力并将容器保持一段时间; 并将容器中的压力降低至环境压力，并将温度升高至22℃-37℃。通过进行这些步骤，细胞比参考物细胞凋亡更少。

公开(公告)号：US10098340B2

公开(公告)日：2018-10-16

申请号：US13982766

申请日：2012-02-03

申请人： William E. Grieshober, Jr. ,  James S. Jones ,  Semyon Kogan ,  Ilya Ilyin ,  Natella I. Enukashvily ,  Yana A. Filkina ,  Alexander N. Shumeev ,  Stanislav Kolchanov

发明人： William E. Grieshober, Jr. ,  James S. Jones ,  Semyon Kogan ,  Ilya Ilyin ,  Natella I. Enukashvily ,  Yana A. Filkina ,  Alexander N. Shumeev ,  Stanislav Kolchanov

IPC分类号： A01N1/02

摘要： Provided is a method for reducing apoptosis in nucleated cells. The method entails holding nucleated cells in a container and adding a gas containing xenon to the container so that the pressure inside the container reaches between 0.5 to 4.0 Atm above ambient pressure; holding the container at between 0.5 to 4.0 Atm above ambient pressure for a period of time during which the temperature in the container is between 22° C. and 37° C.; lowering the temperature in the container to between 0.1° C. and 10° C. while maintaining the pressure of 0.5 to 4.0 Atm above ambient pressure and holding the container for a period of time; and reducing the pressure in the container to ambient pressure and increasing the temperature to 22° C.-37° C. By performing these steps, the cells undergo less apoptosis than a reference.

公开(公告)号：US09743660B2

公开(公告)日：2017-08-29

申请号：US13529024

申请日：2012-06-21

申请人： Ilya Ilyin ,  Semyon Kogan ,  William E. Grieshober, Jr. ,  Igor Kachko ,  Vladimir Vasiliev ,  Stanislav A. Kolchanov ,  Yuriy Punin ,  Mark Bakhrakh ,  Alexander N. Shumeev

发明人： Ilya Ilyin ,  Semyon Kogan ,  William E. Grieshober, Jr. ,  Igor Kachko ,  Vladimir Vasiliev ,  Stanislav A. Kolchanov ,  Yuriy Punin ,  Mark Bakhrakh ,  Alexander N. Shumeev

IPC分类号： A61B19/00 ,  A01N1/02 ,  A61J1/10 ,  A61J1/16

CPC分类号： A01N1/021 ,  A01N1/0242 ,  A01N1/0263 ,  A01N1/0289 ,  A61J1/10 ,  A61J1/16

摘要： A system, method, and device for preserving blood and its components is described. The system and method generally include a device having a body defining a chamber, the chamber being configured to receive at least one bag containing blood or its components, the at least one bag being permeable to gas, for example, xenon. A cover is hermetically sealable to the body. An inlet is in fluid communication with the chamber. A pressure indicator is configured to indicate pressure in the chamber, the pressure indicator including a conduit containing a liquid. A portion of the conduit is transparent such that the liquid is visible. A source of pressurized gas, such as xenon, is provided to provide the pressurized gas to the chamber.

公开(公告)号：US20130157249A1

公开(公告)日：2013-06-20

申请号：US13529024

申请日：2012-06-21

申请人： Ilya Ilyin ,  Semyon Kogan ,  William E. Grieshober, JR. ,  Igor Kachko ,  Vladimir Vasiliev ,  Stanislav A. Kolchanov ,  Yuriy Punin ,  Mark Bakhrakh ,  Alexander N. Shumeev

发明人： Ilya Ilyin ,  Semyon Kogan ,  William E. Grieshober, JR. ,  Igor Kachko ,  Vladimir Vasiliev ,  Stanislav A. Kolchanov ,  Yuriy Punin ,  Mark Bakhrakh ,  Alexander N. Shumeev

IPC分类号： A01N1/02

CPC分类号： A01N1/021 ,  A01N1/0242 ,  A01N1/0263 ,  A01N1/0289 ,  A61J1/10 ,  A61J1/16

摘要： A system, method, and device for preserving blood and its components is described. The system and method generally include a device having a body defining a chamber, the chamber being configured to receive at least one bag containing blood or its components, the at least one bag being permeable to gas, for example, xenon. A cover is hermetically sealable to the body. An inlet is in fluid communication with the chamber. A pressure indicator is configured to indicate pressure in the chamber, the pressure indicator including a conduit containing a liquid. A portion of the conduit is transparent such that the liquid is visible. A source of pressurized gas, such as xenon, is provided to provide the pressurized gas to the chamber.

摘要翻译： 描述了一种用于保存血液及其组分的系统，方法和装置。 该系统和方法通常包括具有限定腔室的主体的装置，所述腔室构造成容纳至少一个含有血液或其组分的袋，所述至少一个袋子可渗透气体，例如氙气。 盖子对身体密封。 入口与腔室流体连通。 压力指示器构造成指示腔室中的压力，压力指示器包括含有液体的导管。 导管的一部分是透明的，使得液体是可见的。 提供诸如氙气的加压气体源，以将加压气体提供给腔室。