公开(公告)号：US20070184072A1

公开(公告)日：2007-08-09

申请号：US11644207

申请日：2006-12-22

申请人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

发明人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

IPC分类号： A61K39/09 ,  C07K14/31

CPC分类号： A61K39/092 ,  A61K39/385 ,  A61K2039/6031 ,  A61K2039/6037

摘要： An immunogenic composition having 13 distinct polysaccharide-protein conjugates and optionally, an aluminum-based adjuvant, is described. Each conjugate contains a capsular polysaccharide prepared from a different serotype of Streptococcus pneumoniae (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) conjugated to a carrier protein. The immunogenic composition, formulated as a vaccine, increases coverage against pneumococcal disease in infants and young children globally, and provides coverage for serotypes 6A and 19A that is not dependent on the limitations of serogroup cross-protection. Also described is a method for making an immunogenic conjugate comprising Streptococcus pneumoniae serotype 1 polysaccharide covalently linked to a carrier protein, the method including partial de-O-acetylation of the polysaccharide by mild hydrolysis in an alkaline pH buffer.

摘要翻译： 描述了具有13种不同的多糖 - 蛋白质缀合物和任选的基于铝的佐剂的免疫原性组合物。 每个缀合物含有由与载体蛋白缀合的不同血清型肺炎链球菌（1,3,4,5,6A，6B，7F，9V，14,18C，19A，19F和23F）制备的荚膜多糖。 配制成疫苗的免疫原性组合增加了全球婴儿和幼儿肺炎球菌病的覆盖率，并为不依赖于血清群交叉保护的限制的血清型6A和19A提供了覆盖。 还描述了一种用于制备包含与载体蛋白共价连接的肺炎链球菌血清型1多糖的免疫原性缀合物的方法，该方法包括在碱性pH缓冲液中通过轻度水解部分脱-O-乙酰化多糖。

公开(公告)号：US20070231340A1

公开(公告)日：2007-10-04

申请号：US11644924

申请日：2006-12-22

申请人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

发明人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

IPC分类号： A61K38/00 ,  C07K14/00

CPC分类号： A61K39/092 ,  A61K2039/55505 ,  A61K2039/6037 ,  A61K2039/70 ,  C07K14/3156

摘要： An immunogenic composition having 13 distinct polysaccharide-protein conjugates and optionally, an aluminum-based adjuvant, is described. Each conjugate contains a capsular polysaccharide prepared from a different serotype of Streptococcus pneumoniae (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) conjugated to a carrier protein. The immunogenic composition, formulated as a vaccine, increases coverage against pneumococcal disease in infants and young children globally, and provides coverage for serotypes 6A and 19A that is not dependent on the limitations of serogroup cross-protection. Also described is a method for making an immunogenic conjugate comprising Streptococcus pneumoniae serotype 3 polysaccharide covalently linked to a carrier protein, the method including periodic acid oxidation of the polysaccharide in the presence of bivalent cations.

摘要翻译： 描述了具有13种不同的多糖 - 蛋白质缀合物和任选的基于铝的佐剂的免疫原性组合物。 每个缀合物含有由与载体蛋白缀合的不同血清型肺炎链球菌（1,3,4,5,6A，6B，7F，9V，14,18C，19A，19F和23F）制备的荚膜多糖。 配制成疫苗的免疫原性组合增加了全球婴儿和幼儿肺炎球菌病的覆盖率，并为不依赖于血清群交叉保护的限制的血清型6A和19A提供了覆盖。 还描述了一种用于制备包含与载体蛋白共价连接的肺炎链球菌血清型3多糖的免疫原性缀合物的方法，该方法包括在二价阳离子存在下对多糖进行高碘酸氧化。

公开(公告)号：US20070184071A1

公开(公告)日：2007-08-09

申请号：US11644095

申请日：2006-12-22

申请人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

发明人： William Hausdorff ,  George Siber ,  Peter Paradiso ,  A. Prasad

IPC分类号： A61K39/09 ,  C07K14/31

CPC分类号： A61K47/646 ,  A61K39/092 ,  A61K2039/55505 ,  A61K2039/6037 ,  A61K2039/70

摘要： An immunogenic composition having 13 distinct polysaccharide-protein conjugates and optionally, an aluminum-based adjuvant, is described. Each conjugate contains a capsular polysaccharide prepared from a different serotype of Streptococcus pneumoniae (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) conjugated to a carrier protein. The immunogenic composition, formulated as a vaccine, increases coverage against pneumococcal disease in infants and young children globally, and provides coverage for serotypes 6A and 19A that is not dependent on the limitations of serogroup cross-protection. Methods for making an immunogenic conjugate comprising Streptococcus pneumoniae serotype 19A polysaccharide are also provided in which the serotype 19A polysaccharide is co-lyophilized with a carrier protein and conjugation is carried out in dimethyl sulfoxide (DMSO) via a reductive amination mechanism.

摘要翻译： 描述了具有13种不同的多糖 - 蛋白质缀合物和任选的基于铝的佐剂的免疫原性组合物。 每个缀合物含有由与载体蛋白缀合的不同血清型肺炎链球菌（1,3,4,5,6A，6B，7F，9V，14,18C，19A，19F和23F）制备的荚膜多糖。 配制成疫苗的免疫原性组合增加了全球婴儿和幼儿肺炎球菌病的覆盖率，并为不依赖于血清群交叉保护的限制的血清型6A和19A提供了覆盖。 还提供了制备包含肺炎链球菌血清型19A多糖的免疫原性缀合物的方法，其中血清型19A多糖与载体蛋白共冻干，并通过还原胺化机制在二甲基亚砜（DMSO）中进行偶联。

公开(公告)号：US20060228380A1

公开(公告)日：2006-10-12

申请号：US11395593

申请日：2006-03-31

申请人： William Hausdorff ,  George Siber ,  Peter Paradiso

发明人： William Hausdorff ,  George Siber ,  Peter Paradiso

IPC分类号： A61K39/09

摘要： An immunogenic composition having 13 distinct polysaccharide-protein conjugates and optionally, an aluminum-based adjuvant, is described. Each conjugate contains a capsular polysaccharide prepared from a different serotype of Streptococcus pneumoniae (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F) conjugated to a carrier protein. The immunogenic composition, formulated as a vaccine, increases coverage against pneumococcal disease in infants and young children globally, and provides coverage for serotypes 6A and 19A that is not dependent on the limitations of serogroup cross-protection.

摘要翻译： 描述了具有13种不同的多糖 - 蛋白质缀合物和任选的基于铝的佐剂的免疫原性组合物。 每个缀合物含有由与载体蛋白缀合的不同血清型肺炎链球菌（1,3,4,5,6A，6B，7F，9V，14,18C，19A，19F和23F）制备的荚膜多糖。 配制成疫苗的免疫原性组合增加了全球婴儿和幼儿肺炎球菌病的覆盖率，并为不依赖于血清群交叉保护的限制的血清型6A和19A提供了覆盖。