公开(公告)号：US20180265858A1

公开(公告)日：2018-09-20

申请号：US15912165

申请日：2018-03-05

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Glauco R. SOUZA ,  Renata Pasqualini ,  Wadih ARAP ,  Thomas Charles KILLIAN ,  Robert M. RAPHAEL ,  Daniel Joshua STARK

IPC分类号： C12N13/00 ,  C12M1/42 ,  B82Y5/00 ,  C12N5/00

CPC分类号： C12N13/00 ,  B82Y5/00 ,  C12M35/06 ,  C12N5/0062 ,  C12N2529/00 ,  C12N2533/10

摘要： Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

公开(公告)号：US20140322784A1

公开(公告)日：2014-10-30

申请号：US14331377

申请日：2014-07-15

申请人： William Marsh Rice University

发明人： Glauco R. SOUZA ,  Renata Pasqualini ,  Wadih ARAP ,  Thomas Charles KILLIAN ,  Robert M. RAPHAEL ,  Daniel Joshua STARK

IPC分类号： C12N13/00 ,  C12N5/00

CPC分类号： C12N13/00 ,  B82Y5/00 ,  C12M35/06 ,  C12N5/0062 ,  C12N2529/00 ,  C12N2533/10

摘要： Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

摘要翻译： 讨论了一般在医学，细胞生物学，纳米技术和细胞培养中有用的系统和方法。 特别地，至少在一些实施例中，讨论了用于细胞和材料的磁性引导和图案化的系统和方法。 这些系统和方法的一些具体应用可以包括将细胞悬浮培养离开表面，制备和操纵悬浮细胞的图案，以及对表面上的细胞进行图案化培养。 具体地说，提出培养细胞的方法。 该方法可以包括提供多个单元，提供磁场，以及悬浮在磁场中的多个单元中的至少一些单元，其中多个单元包括磁性纳米颗粒。 该方法还可以包括将悬浮液维持足以允许细胞生长形成组件的时间。