公开(公告)号：US20230183723A1

公开(公告)日：2023-06-15

申请号：US17926113

申请日：2021-05-18

Applicant: Wisconsin Alumni Research Foundation

Inventor： Shaoqin GONG ,  Xuehua Zhong ,  Yuyuan Wang ,  Shuiming Qian

IPC: C12N15/82 ,  C08G77/392 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/8206 ,  C08G77/392 ,  C12N15/8213 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20

Abstract: The present technology provides a nanoparticle that includes a silica network comprising crosslinked polysiloxanes, wherein the crosslinks comprise disulfide linkages, and the nanoparticle has a surface bearing charged functional groups and a surface potential of either less than −30 mV or greater than +30 mV, and wherein the nanoparticle has an average diameter of 20 nm to 60 n. The nanoparticles may be used to efficiently deliver biomolecules to plant cells, including polynucleic acids, proteins and complexes thereof (e.g., Cas9 RNP).