公开(公告)号：US11485964B2

公开(公告)日：2022-11-01

申请号：US16931967

申请日：2020-07-17

Applicant: Worcester Polytechnic Institute

Inventor： Amy Peterson ,  Marsha Rolle ,  Ivan Ding

IPC: C12N13/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/00

Abstract: Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.

公开(公告)号：US10731145B2

公开(公告)日：2020-08-04

申请号：US15380374

申请日：2016-12-15

Applicant: Worcester Polytechnic Institute

Inventor： Amy Peterson ,  Marsha Rolle ,  Ivan Ding

IPC: C12N13/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/00

Abstract: Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.

公开(公告)号：US20210171931A1

公开(公告)日：2021-06-10

申请号：US16931967

申请日：2020-07-17

Applicant: Worcester Polytechnic Institute

Inventor： Amy Peterson ,  Marsha Rolle ,  Ivan Ding

IPC: C12N13/00 ,  C12N5/00 ,  C12M1/12 ,  C12M3/00 ,  C12M1/00

Abstract: Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.

公开(公告)号：US20170166884A1

公开(公告)日：2017-06-15

申请号：US15380374

申请日：2016-12-15

Applicant: Worcester Polytechnic Institute

Inventor： Amy Peterson ,  Marsha Rolle ,  Ivan Ding

IPC: C12N13/00 ,  C12N5/00 ,  C12M1/12 ,  C12M1/00 ,  C12M3/00

Abstract: Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.