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1.发明申请Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports 有权用于在固体支持物上优化寡核苷酸合成和纯化的接头和共偶联剂公开(公告)号：US20070287832A1
公开(公告)日：2007-12-13
申请号：US11726269
申请日：2007-03-21
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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2.发明申请Linkers and Co-Coupling Agents for Optimization of Oligonucleotide Synthesis and Purification on Solid Supports 有权用于优化固体支持物上的寡核苷酸合成和纯化的接头和共偶联剂公开(公告)号：US20100093974A1
公开(公告)日：2010-04-15
申请号：US12493985
申请日：2009-06-29
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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3.发明授权Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports 有权用于在固体支持物上优化寡核苷酸合成和纯化的接头和共偶联剂公开(公告)号：US08053187B2
公开(公告)日：2011-11-08
申请号：US12897483
申请日：2010-10-04
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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4.发明授权Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports 有权用于在固体支持物上优化寡核苷酸合成和纯化的接头和共偶联剂公开(公告)号：US07807807B2
公开(公告)日：2010-10-05
申请号：US12493985
申请日：2009-06-29
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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5.发明授权Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports 有权用于在固体支持物上优化寡核苷酸合成和纯化的接头和共偶联剂公开(公告)号：US07553958B2
公开(公告)日：2009-06-30
申请号：US11726269
申请日：2007-03-21
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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6.发明申请LINKERS AND CO-COUPLING AGENTS FOR OPTIMIZATION OF OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION ON SOLID SUPPORTS 有权用于优化寡核苷酸合成和固相支持物的连接剂和偶联剂公开(公告)号：US20120029166A1
公开(公告)日：2012-02-02
申请号：US13270865
申请日：2011-10-11
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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7.发明申请LINKERS AND CO-COUPLING AGENTS FOR OPTIMIZATION OF OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION ON SOLID SUPPORTS 有权用于优化寡核苷酸合成和固相支持物的连接剂和偶联剂公开(公告)号：US20110097762A1
公开(公告)日：2011-04-28
申请号：US12897483
申请日：2010-10-04
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
IPC分类号： C12P19/34
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
摘要翻译： 描述了从固体支持物调节合成低聚物的合成能力和裂解性质的方法。 该方法利用连接到固体表面的连接分子和与表面官能团的偶联化合物具有相似反应性的共偶联剂。 优选的接头分子提供了增加的聚合物密度，并且具有更强的抗支撑表面的裂解性。 该方法对于合成寡核苷酸，寡核苷酸微阵列，肽和肽微阵列特别有用。 稳定的接头还与锚分子偶联，用于在支持物纯化上合成DNA寡核苷酸，消除了耗时的色谱和金属阳离子存在。 如此获得的寡核苷酸可以直接用于质量分析，DNA扩增和连接，杂交和许多其他应用。
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公开(公告)号：US07211654B2
公开(公告)日：2007-05-01
申请号：US10099382
申请日：2002-03-13
申请人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
发明人： Xiaolian Gao , Hua Zhang , Peilin Yu , Eric Leproust , Jean Philippe Pellois , Qin Xiang , Xiaochuan Zhou
CPC分类号： C07H19/06 , C07B2200/11 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07K2/00 , C40B40/06 , C40B50/18 , C40B80/00
摘要： A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
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公开(公告)号：US20110288285A1
公开(公告)日：2011-11-24
申请号：US13065467
申请日：2011-03-22
申请人： Xiaolian Gao , Peilin Yu
发明人： Xiaolian Gao , Peilin Yu
CPC分类号： C07F9/65586 , C07F9/2408 , C07F9/2458 , C07H21/04
摘要： The present invention describes novel compounds and methods for capping reactive groups on support and during multistep synthesis. These new capping reagents are also useful for high quality synthesis on solid supports and surfaces used as microarrays, biosensors, or in general as biochips. The compounds are also useful for controlling surface density of reactive groups on a support. The compounds may also be used to modify the hydrophilic/hydrophobic characteristics of a surface or a molecule. The compounds have functional utility in various applications in the fields of genomics, proteomics, diagnostics and medicine.
摘要翻译： 本发明描述了新型化合物和用于在载体上和在多步合成中封端反应性基团的方法。 这些新的封端试剂也可用于固体支持物和用作微阵列，生物传感器或一般作为生物芯片的表面上的高质量合成。 这些化合物也可用于控制载体上反应性基团的表面密度。 化合物也可用于改变表面或分子的亲水/疏水特性。 该化合物在基因组学，蛋白质组学，诊断学和医学领域的各种应用中具有功能实用性。
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公开(公告)号：US07923550B2
公开(公告)日：2011-04-12
申请号：US10553328
申请日：2004-04-14
申请人： Xiaolian Gao , Peilin Yu
发明人： Xiaolian Gao , Peilin Yu
IPC分类号： C07H21/00
CPC分类号： C07F9/2408
摘要： The present invention describes novel compounds and methods for capping reactive groups on support and during multistep synthesis. These new capping reagents are also useful for high quality synthesis on solid supports and surfaces used as microarrays, biosensors, or in general as biochips. The compounds are also useful for controlling surface density of reactive groups on a support. The compounds may also be used to modify the hydrophilic/hydrophobic characteristics of a surface or a molecule. The compounds have functional utility in various applications in the fields of genomics, proteomics, diagnostics and medicine.
摘要翻译： 本发明描述了新型化合物和用于在载体上和在多步合成中封端反应性基团的方法。 这些新的封端试剂也可用于固体支持物和用作微阵列，生物传感器或一般作为生物芯片的表面上的高质量合成。 这些化合物也可用于控制载体上反应性基团的表面密度。 化合物也可用于改变表面或分子的亲水/疏水特性。 该化合物在基因组学，蛋白质组学，诊断学和医学领域的各种应用中具有功能实用性。
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