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    Yellow pigments generation deficient Sphingomonas strain and application thereof in gellan gum production
    1.
    发明授权
    Yellow pigments generation deficient Sphingomonas strain and application thereof in gellan gum production 有权
    黄色色素生成缺陷型鞘氨醇单胞菌菌株及其在结冷胶生产中的应用

    公开(公告)号：US08685698B2

    公开(公告)日：2014-04-01

    申请号：US13142412

    申请日：2010-08-13

    申请人： Xuechang Wu ,  Rongming Wu ,  Ou Li ,  Liang Zhu ,  Yamin Chen ,  Chaodong Qian ,  Mei Chen

    发明人： Xuechang Wu ,  Rongming Wu ,  Ou Li ,  Liang Zhu ,  Yamin Chen ,  Chaodong Qian ,  Mei Chen

    IPC分类号： C12N1/12 ,  C12P19/04

    CPC分类号： C12R1/01 ,  C12P19/04

    摘要： A yellow pigments generation deficient Sphingomonas strain (Sphingomonas sp. ZD001) and application thereof in preparing gellan gum by microbial fermentation are provided. The strain is preserved in China Center for Type Culture Collection (CCTCC) with the address of Wuhan University, Wuhan, 430072, China, the preservation date of the strain is 10 Sep. 2009, and the preservation serial number is CCTCC No: M 209298. The main beneficial effect of the present invention is that the fermented liquor contains no yellow pigments but is milky white, colorless superior gellan gum can be obtained by only depositing polysaccharide with a small quantity of ethanol or isopropanol, so that the post purification and de-coloration techniques of gellan gum production can be simplified, the yield can be enhanced, and the production cost can be reduced.

    摘要翻译： 提供黄色色素代谢缺陷型鞘氨醇单胞菌（Sphingomonas sp。ZD001）及其通过微生物发酵制备吉兰糖胶的应用。 该菌株保藏在中国类型培养物保藏中心（CCTCC），武汉大学地址，武汉，430072，保护期为2009年9月10日，保藏号为CCTCC号：209198 本发明的主要有益效果在于，发酵液不含黄色颜料，但为乳白色，仅通过少量乙醇或异丙醇沉淀多糖即可获得无色的优质结冷胶，因此后期纯化和脱色 可以简化结冷胶生产的成色技术，可以提高产量，降低生产成本。

    Yellow Pigments Generation Deficient Sphingomonas Strain and Application Thereof in Gellan Gum Production
    2.
    发明申请
    Yellow Pigments Generation Deficient Sphingomonas Strain and Application Thereof in Gellan Gum Production 有权
    黄色素生成缺乏的鞘氨醇单胞菌菌株及其在结冷胶生产中的应用

    公开(公告)号：US20110281308A1

    公开(公告)日：2011-11-17

    申请号：US13142412

    申请日：2010-08-13

    申请人： Xuechang Wu ,  Rongming Wu ,  Ou Li ,  Liang Zhu ,  Yamin Chen ,  Chaodong Qian ,  Mei Chen

    发明人： Xuechang Wu ,  Rongming Wu ,  Ou Li ,  Liang Zhu ,  Yamin Chen ,  Chaodong Qian ,  Mei Chen

    IPC分类号： C12P19/04 ,  C12N1/20

    CPC分类号： C12R1/01 ,  C12P19/04

    摘要： A yellow pigments generation deficient Sphingomonas strain (Sphingomonas sp. ZD001) and application thereof in preparing gellan gum by microbial fermentation are provided. The strain is preserved in China Center for Type Culture Collection (CCTCC) with the address of Wuhan University, Wuhan, 430072, China, the preservation date of the strain is 10 Sep. 2009, and the preservation serial number is CCTCC No: M 209298. The main beneficial effect of the present invention is that the fermented liquor contains no yellow pigments but is milky white, colorless superior gellan gum can be obtained by only depositing polysaccharide with a small quantity of ethanol or isopropanol, so that the post purification and de-coloration techniques of gellan gum production can be simplified, the yield can be enhanced, and the production cost can be reduced.

    摘要翻译： 提供黄色色素代谢缺陷型鞘氨醇单胞菌（Sphingomonas sp。ZD001）及其通过微生物发酵制备吉兰糖胶的应用。 该菌株保藏在中国类型培养物保藏中心（CCTCC），武汉大学地址，武汉，430072，保护期为2009年9月10日，保藏号为CCTCC号：209198 本发明的主要有益效果在于，发酵液不含黄色颜料，但为乳白色，仅通过少量乙醇或异丙醇沉淀多糖即可获得无色的优质结冷胶，因此后期纯化和脱色 可以简化结冷胶生产的成色技术，可以提高产量，降低生产成本。

    Method for post-extracting low acyl gellan gum with high solubility
    4.
    发明授权
    Method for post-extracting low acyl gellan gum with high solubility 有权
    高溶解度的低酰基结冷胶的萃取方法

    公开(公告)号：US08652549B2

    公开(公告)日：2014-02-18

    申请号：US13142407

    申请日：2010-06-02

    申请人： Rongming Wu ,  Yanfei Zhong ,  Qi Guo ,  Yueqiang Shen ,  Liping Shen

    发明人： Rongming Wu ,  Yanfei Zhong ,  Qi Guo ,  Yueqiang Shen ,  Liping Shen

    IPC分类号： C12P19/04 ,  A23L1/054 ,  C08B37/00

    CPC分类号： C12P19/04 ,  C08B37/0003 ,  C08B37/006

    摘要： The invention relates to a post-extraction process for preparing low acyl gellan gum having high solubility property, the process comprising the following steps: (1) enzyme treatment of fermentation broth; (2) flocculation of fermentation broth; (3) deacylation treatment; (4) clarification treatment of deacylated solution; (5) dehydration treatment of clarified and deacylated gellan gum solution; (6) ion exchange and decoloration treatment; and (7) drying and milling. The process according to the invention produces a product, which has largely improved solubility, largely improved quality, good appearance, high transparence, and high gel strength.

    摘要翻译： 本发明涉及一种制备具有高溶解性的低酰基结冷胶的后萃取方法，该方法包括以下步骤：（1）发酵液的酶处理; （2）发酵液的絮凝; （3）脱酰基处理; （4）脱酰基溶液的澄清处理; （5）脱水处理澄清和脱酰基吉兰糖胶溶液; （6）离子交换和脱色处理; 和（7）干燥和研磨。 根据本发明的方法产生了大大改善溶解度，大大提高质量，良好的外观，高透明度和高凝胶强度的产品。

    Method for Post-Extracting Low Acyl Gellan Gum with High Solubility
    5.
    发明申请
    Method for Post-Extracting Low Acyl Gellan Gum with High Solubility 有权
    以高溶解度萃取低酰基凝胶的方法

    公开(公告)号：US20110268843A1

    公开(公告)日：2011-11-03

    申请号：US13142407

    申请日：2010-06-02

    申请人： Rongming Wu ,  Yanfei Zhong ,  Qi Guo ,  Yueqiang Shen ,  Liping Shen

    发明人： Rongming Wu ,  Yanfei Zhong ,  Qi Guo ,  Yueqiang Shen ,  Liping Shen

    IPC分类号： A23L1/054

    CPC分类号： C12P19/04 ,  C08B37/0003 ,  C08B37/006

    摘要： The invention relates to a post-extraction process for preparing low acyl gellan gum having high solubility property, the process comprising the following steps: (1) enzyme treatment of fermentation broth; (2) flocculation of fermentation broth; (3) deacylation treatment; (4) clarification treatment of deacylated solution; (5) dehydration treatment of clarified and deacylated gellan gum solution; (6) ion exchange and decoloration treatment; and (7) drying and milling. The process according to the invention produces a product, which has largely improved solubility, largely improved quality, good appearance, high transparence, and high gel strength.

    摘要翻译： 本发明涉及一种制备具有高溶解性的低酰基结冷胶的后萃取方法，该方法包括以下步骤：（1）酶处理发酵液; （2）发酵液的絮凝; （3）脱酰基处理; （4）脱酰基溶液的澄清处理; （5）脱水处理澄清和脱酰基吉兰糖胶溶液; （6）离子交换和脱色处理; 和（7）干燥和研磨。 根据本发明的方法产生了大大改善溶解度，大大提高质量，良好的外观，高透明度和高凝胶强度的产品。