公开(公告)号：US20100304470A1

公开(公告)日：2010-12-02

申请号：US12497781

申请日：2009-07-06

Applicant: YA-FU CHUANG ,  I-EN LIN

Inventor： YA-FU CHUANG ,  I-EN LIN

IPC: C12M1/34

CPC classification number: G01N33/5304 ,  B01L3/502738 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0688

Abstract: A microfluidic chip including a substrate and at least a channel set is provided. The substrate has a surface, and the channel set is formed in the substrate and includes a channel, at a least a filler fillister, and at least a well fillister. The filler fillister and the well fillister are all connected to the channel, and the channel passes through the well fillister. The depth of the well fillister relative to the surface is longer than the depth of the channel relative to the surface. Hence, the well fillister can hinder the fluid flow in the channel and be used as a valve.

Abstract translation: 提供了包括基板和至少一个通道组的微流体芯片。 衬底具有表面，并且沟道组形成在衬底中，并且包括至少填充物填充物的通道和至少填充阱。 填料填充器和注油器都连接到通道，并且通道通过井填充。 阱填充器相对于表面的深度比通道相对于表面的深度长。 因此，填充液可能妨碍通道中的流体流动并用作阀门。

公开(公告)号：US20130109036A1

公开(公告)日：2013-05-02

申请号：US13284812

申请日：2011-10-28

Applicant: YI-LING YE ,  LI-SHIAN SHI ,  LIN-YI WU ,  I-EN LIN ,  JENG-HAUR HORNG ,  L. JAMES LEE

Inventor： YI-LING YE ,  LI-SHIAN SHI ,  LIN-YI WU ,  I-EN LIN ,  JENG-HAUR HORNG ,  L. JAMES LEE

IPC: G01N33/53 ,  C12P21/00

CPC classification number: G01N33/54353

Abstract: A protein substrate includes a base having micro-passages and reaction grooves, a polyethyleneimine (PEI) fixed on the base and a specific protein fixed on the PEI. The base is modified by plasma stripper to make the PEI bonded on the base. Mixed with tyrosinase, the specific protein can stably stick on the base due to the tyrosinase bonded with the PEI. By antigen-antibody bonding specificity, the invention can quickly detect antibody in a sample able to link with the specific protein. The specific antibody can be added with targeted biotin secondary antibody, targeted avidin-peroxidase, and color producing substrate able to react with the targeted avidin-peroxidase, such as tetramethylbenzidine or 3-(4-hydroxy)phenyl propionic acid (HPPA), so as to measure a specific antibody amount.

Abstract translation: 蛋白质底物包括具有微通道和反应槽的基底，固定在基底上的聚乙烯亚胺（PEI）和固定在PEI上的特定蛋白质。 基体由等离子体汽提器改性，使PEI结合在基体上。 与酪氨酸酶混合，由于与PEI结合的酪氨酸酶，特异性蛋白质可以稳定地粘在基质上。 通过抗原 - 抗体结合特异性，本发明可以快速检测能够与特异性蛋白质连接的样品中的抗体。 特异性抗体可以加入靶向的生物素二抗，靶向的抗生物素蛋白 - 过氧化物酶和能够与靶向的抗生物素蛋白 - 过氧化物酶如四甲基联苯胺或3-（4-羟基）苯基丙酸（HPPA）反应的产生色素的底物，因此 以测量特异性抗体量。