公开(公告)号：US20220217934A1

公开(公告)日：2022-07-14

申请号：US17272685

申请日：2019-09-05

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Maxim ITKIN ,  Adam JOZWlAK

IPC: A01H6/82 ,  C12N15/82 ,  C12N9/10

Abstract: Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.

公开(公告)号：US20240150744A1

公开(公告)日：2024-05-09

申请号：US18281341

申请日：2022-03-10

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Adam JOZWIAK ,  Paula BERMAN ,  Luis DE-HARO

IPC: C12N9/00 ,  C12N15/82 ,  C12P17/18

CPC classification number: C12N9/93 ,  C12N15/8243 ,  C12P17/182

Abstract: The present invention provides polynucleotide sequences derived from Helichrysum umbraculigerum and encoding a protein or a plurality thereof belonging to the acyl activating enzyme (AAE) family. Further provided are an artificial nucleic acid molecule including the polynucleotide, a transgenic cell, tissue, or plant including same. Further provided are method for synthesizing an acyl coenzyme A (CoA).

公开(公告)号：US20250092415A1

公开(公告)日：2025-03-20

申请号：US18910862

申请日：2024-10-09

Applicant: Yeda Research and Development Co. Ltd. ,  Yissum Research Development Company of The Hebrew University of Jerusalem Ltd.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Pablo D. CARDENAS ,  Saul BURDMAN

IPC: C12N15/82 ,  C12N9/02 ,  C12N9/04

Abstract: Disclosed herein are genetically modified plants having altered biological activity of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Methods of producing these genetically modified plants are also disclosed.

公开(公告)号：US20240315192A1

公开(公告)日：2024-09-26

申请号：US18735258

申请日：2024-06-06

Applicant: YEDA RESEARCH AND DEVELOPMENT CO., LTD.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Maxim ITKIN ,  Adam JOZWIAK

IPC: A01H6/82 ,  C12N9/10 ,  C12N15/82

CPC classification number: A01H6/82 ,  C12N9/1059 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8247

Abstract: Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.

公开(公告)号：US20240182873A1

公开(公告)日：2024-06-06

申请号：US18281536

申请日：2022-03-10

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Adam JOZWIAK ,  Paula BERMAN ,  Luis DE-HARO

IPC: C12N9/10 ,  C12P7/42

CPC classification number: C12N9/1085 ,  C12P7/42

Abstract: The present invention provides polynucleotide sequences derived from Helichrysum umbraculigerum and encoding a protein or a plurality thereof belonging to the prenyltransferase (PT) family. Further provided are an artificial nucleic acid molecule including the polynucleotide, a transgenic cell, tissue, or plant including same.

公开(公告)号：US20220408679A1

公开(公告)日：2022-12-29

申请号：US17859053

申请日：2022-07-07

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Asaph AHARONI ,  Prashant SONAWANE ,  Maxim ITKIN

IPC: A01H6/82 ,  C12N15/82 ,  C12N9/10

Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.