公开(公告)号：US07332592B2

公开(公告)日：2008-02-19

申请号：US11144747

申请日：2005-06-03

申请人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

发明人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

IPC分类号： C07H21/02 ,  C07H21/04

CPC分类号： C07K14/70596

摘要： Studies in mice and humans indicate that membrane CD14 (mCD14) on the cell surface of monocytes, macrophages, and PMN mediates the activation of these cells by LPS. The soluble CD14 (sCD14) present in the circulation also binds to LPS and blocks LPS binding to mCD14. To determine the role of a recombinant bovine soluble CD14 polypeptide in cellular activation by LPS, a recombinant bovine soluble CD14 polypeptide, rbosCD14, was cloned and expressed in a baculovirus expression system. Results indicated that rbosCD14 inhibited the LPS-induced increase in CD18 expression and TNFα mRNA in vitro and reduced mortality in mice injected with LPS. Further, rbosCD14 sensitized mammary epithelial cells to low concentrations of LPS resulting in recruitment of white blood cells and prevention of LPS-induced infection.

摘要翻译： 小鼠和人类的研究表明，单核细胞，巨噬细胞和PMN的细胞表面上的膜CD14（mCD14）介导LPS对这些细胞的活化。 存在于循环中的可溶性CD14（sCD14）还结合LPS并阻断LPS与mCD14的结合。 为了确定重组牛可溶性CD14多肽在LPS细胞活化中的作用，将重组牛可溶性CD14多肽rbosCD14克隆并表达在杆状病毒表达系统中。 结果表明，rbosCD14抑制LPS诱导的体外CD18表达和TNFαmRNA的增加，降低LPS注射小鼠的死亡率。 此外，rbosCD14使乳腺上皮细胞对低浓度的LPS敏感，导致白血球募集和预防LPS诱导的感染。

公开(公告)号：US07361351B1

公开(公告)日：2008-04-22

申请号：US11144744

申请日：2005-06-06

申请人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

发明人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

IPC分类号： A61K39/00 ,  G01N33/53

CPC分类号： C07K14/70596

摘要： Studies in mice and humans indicate that membrane CD14 (mCD14) on the cell surface of monocytes, macrophages, and PMN mediates the activation of these cells by LPS. The soluble CD14 (sCD14) present in the circulation also binds to LPS and blocks LPS binding to mCD14. To determine the role of a recombinant bovine soluble CD14 polypeptide in cellular activation by LPS, a recombinant bovine soluble CD14 polypeptide, rbosCD14, was cloned and expressed in a baculovirus expression system. Results indicated that rbosCD14 inhibited the LPS-induced increase in CD18 expression and TNFα mRNA in vitro and reduced mortality in mice injected with LPS. Further, rbosCD14 sensitized mammary epithelial cells to low concentrations of LPS resulting in recruitment of white blood cells and prevention of LPS-induced infection.

摘要翻译： 小鼠和人类的研究表明，单核细胞，巨噬细胞和PMN的细胞表面上的膜CD14（mCD14）介导LPS对这些细胞的活化。 存在于循环中的可溶性CD14（sCD14）还结合LPS并阻断LPS与mCD14的结合。 为了确定重组牛可溶性CD14多肽在LPS细胞活化中的作用，将重组牛可溶性CD14多肽rbosCD14克隆并表达在杆状病毒表达系统中。 结果表明，rbosCD14抑制LPS诱导的体外CD18表达和TNFαmRNA的增加，降低LPS注射小鼠的死亡率。 此外，rbosCD14使乳腺上皮细胞对低浓度的LPS敏感，导致白血球募集和预防LPS诱导的感染。

公开(公告)号：US06984503B1

公开(公告)日：2006-01-10

申请号：US10184005

申请日：2002-06-27

申请人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

发明人： Yan Wang ,  Dante S. Zarlenga ,  Max J. Paape

IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C07K14/70596

摘要： Studies in mice and humans indicate that membrane CD14 (mCD14) on the cell surface of monocytes, macrophages, and PMN mediates the activation of these cells by LPS. The soluble CD14 (sCD14) present in the circulation also binds to LPS and blocks LPS binding to mCD14. To determine the role of a recombinant bovine soluble CD14 polypeptide in cellular activation by LPS, a recombinant bovine soluble CD14 polypeptide, rbosCD14, was cloned and expressed in a baculovirus expression system. Results indicated that rbosCD14 inhibited the LPS-induced increase in CD18 expression and TNFα mRNA in vitro and reduced mortality in mice injected with LPS. Further, rbosCD14 sensitized mammary epithelial cells to low concentrations of LPS resulting in recruitment of white blood cells and prevention of LPS-induced infection.

公开(公告)号：US07045510B2

公开(公告)日：2006-05-16

申请号：US10895797

申请日：2004-07-21

申请人： Max J. Paape

发明人： Max J. Paape

IPC分类号： A61K31/721

CPC分类号： A61K31/721

摘要： A method of preventing or treating mastitis in a mammal, involving administering to the mammal a therapeutically effective amount of diethylaminoethyl-dextran (DEAE-dextran) or dextran.