公开(公告)号：US4419349A

公开(公告)日：1983-12-06

申请号：US266038

申请日：1981-05-22

申请人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Yoshimoto Sano ,  Nobuyuki Shibukawa ,  Takashi Hashimoto

发明人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Yoshimoto Sano ,  Nobuyuki Shibukawa ,  Takashi Hashimoto

IPC分类号： A61K36/535 ,  C07C7/00 ,  C07G99/00 ,  A61K35/78

CPC分类号： A61K36/535

摘要： An interferon inducer isolated from the plant tissue, having an elemental analysis of H: 8.5-8.7 %, C: 48.8-49 %, N: 6.3-6.5 % and P: 1.0-1.1 % and a molecular weight of from about 100,000 to about 3,000,000 (mainly about 200,000 to about 1,000,000) and containing as main constituents amino acids, sugars and phosphoric acid. This substance is believed to be a homogeneous polymer of protein and sugars, containing phosphoric acid. This substance has an excellent interferon inducing activity and low toxicity and is significantly active against various viruses. The interferon inducer may be produced by extracting a substance having interferon inducing activity from the tissue of a plant of the genus Perilla of the family Labiatae or a variant thereof containing said active substance and recovering the active substance from the extract thereby obtained. Preferably, the extraction may be effected with water and the recovery may be effected by ultrafiltration. Pharmaceutical composition of this invention comprises as active ingredient the interferon inducer of this invention in association with a pharmaceutical carrier or excipient.

摘要翻译： 从植物组织分离的干扰素诱导物，其H分析为8.5-8.7％，C：48.8-49％，N：6.3-6.5％，P：1.0-1.1％，分子量为约100,000至 约300万（主要为约20万至约100万），主要成分为氨基酸，糖和磷酸。 该物质被认为是含有磷酸的蛋白质和糖的均匀聚合物。 该物质具有优异的干扰素诱导活性和低毒性，对各种病毒具有显着的活性。 干扰素诱导剂可以通过从Labiatae家族的紫苏属植物的组织或其含有所述活性物质的变体的组织提取具有干扰素诱导活性的物质，并从由此获得的提取物中回收活性物质来制备。 优选地，提取可以用水进行，并且可以通过超滤实现回收。 本发明的药物组合物与药物载体或赋形剂一起包含本发明的干扰素诱导剂作为活性成分。

公开(公告)号：US4421746A

公开(公告)日：1983-12-20

申请号：US392994

申请日：1982-06-28

申请人： Yasuhiko Kojima ,  Sadao Tamamura ,  Seishi Konno ,  Takashi Hashimoto

发明人： Yasuhiko Kojima ,  Sadao Tamamura ,  Seishi Konno ,  Takashi Hashimoto

IPC分类号： A61K36/42 ,  A61K36/428 ,  A61K35/78

CPC分类号： A61K36/42 ,  A61K36/428 ,  Y10S514/889

摘要： An interferon inducer is produced by extracting a tissue of a plant belonging to the Gound family selected from the group consisting of Benincasa, Cucurbita, Citrullus, Cucumia, Lagenaria, Luffa, Mormordica, Tricsanthes and their variants by water extraction at a temperature of from ambient to the boiling point of the extraction mixture, forming a supernatant from the extracted solution, fractionating the supernatant to yield fractions containing the interferon inducer and recovering same.

摘要翻译： 干扰素诱导剂是通过在环境温度下通过水提取提取属于野生型家族的植物的组织，所述植物组织选自Benincasa，Cucurbita，Citrullus，Cucumia，Lagenaria，Luffa，Mormordica，Tricsanthes及其变体 到提取混合物的沸点，从提取的溶液形成上清液，分馏上清液以产生含有干扰素诱导物的级分并回收。

公开(公告)号：US4442087A

公开(公告)日：1984-04-10

申请号：US282468

申请日：1981-07-13

申请人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Takashi Hashimoto ,  Nobuyuki Shibukawa

发明人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Takashi Hashimoto ,  Nobuyuki Shibukawa

IPC分类号： C07G99/00 ,  A61K36/28 ,  A61K36/282 ,  A61P43/00 ,  C07K14/415 ,  A61K31/69 ,  A61K39/00 ,  A61K45/02

CPC分类号： C07K14/415 ,  Y10S514/889

摘要： An interferon inducer isolated from the plant tissue, having an elemental analysis of H:7.4.+-.0.4%, C: 45.6.+-.0.4%, N: 13.5.+-.0.4%, P: 2.8.+-.0.3% and a molecular weight of from about 100,000 to about 3,000,000 (mainly about 200,000 to about 1,000,000) and containing as main chemical constituents amino acids and phosporic acid. This substance is believed to be a homogeneous polymer of protein and phosphoric acid.This substance has an excellent interferon inducing activity and low toxicity and is significantly active upon various viruses. This interferon inducer may be produced by extracting a substance having interferon inducing activity from the tissue of a plant of the genus Artemisia of the family Compositae or a variant thereof containing the said active substance with water and recovering the active substance from the extracted solution. The recovery may preferably be effected by ultrafiltration. Pharmaceutical composition of this invention comprises as active ingredient the said active substance in association with a pharmaceutical carrier or excipient.

摘要翻译： 从植物组织分离的干扰素诱导物，具有H：7.4 +/- 0.4％，C：45.6 +/- 0.4％，N：13.5 +/- 0.4％，P：2.8 +/- 0.3％的元素分析和 分子量为约100,000至约3,000,000（主要为约200,000至约1,000,000），并含有主要化学成分的氨基酸和磷酸。 该物质被认为是蛋白质和磷酸的均匀聚合物。 该物质具有优异的干扰素诱导活性和低毒性，对各种病毒具有显着的活性。 这种干扰素诱导剂可以通过从菊科的蒿属植物的组织或其含有所述活性物质的变体的组织中提取具有干扰素诱导活性的物质，并从提取的溶液中回收活性物质来制备。 回收率优选通过超滤来实现。 本发明的药物组合物与药物载体或赋形剂一起包含作为活性成分的所述活性物质。

公开(公告)号：US4469685A

公开(公告)日：1984-09-04

申请号：US491844

申请日：1983-05-05

申请人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Takashi Hashimoto

发明人： Yasuhiko Kojima ,  Seishi Konno ,  Sadao Tamamura ,  Takashi Hashimoto

IPC分类号： A61K36/00 ,  A61K36/233 ,  A61K36/489 ,  A61K36/754 ,  A61K36/8888 ,  A61K36/8965 ,  A61K35/78

CPC分类号： A61K36/00 ,  A61K36/233 ,  A61K36/489 ,  A61K36/754 ,  A61K36/8888 ,  A61K36/8965

摘要： A process for producing interferon inducers from the plants, comprising extracting a water-soluble interferon inducer with water from the tissue of a plant selected from the genera Atractylodes, Lonicera, Plantago, Lithospermum, Ligusticum, Cnidium, Bupleurum, Notopeterygium, Heracleum, Aralia, Panax, Polygala, Sophora, Euchresta, Astragalus, Sinomenium, Stephania, Cocculus, Cimicifuga, Rheum, Gastroida, Asparagus, Pinellia, Evoida and variants thereof capable of producing said interferon inducer at a temperature of from ambient to the boiling point of the extraction mixture for a period sufficient to extract the major portion of said interferon inducer present in the plant tissue, forming a supernatant, fractionating the supernatant to yield fractions containing the major portion of said interferon inducer in the supernatant and recovering said interferon inducer therefrom. Preferably, the fractionation may be effected by ultrafiltration. The interferon inducers thus obtained have a molecular weight of from about 30,000 to about 3,000,000 and are of potential interest for preventing and treating various diseases of humans and animals caused by viral infection.

摘要翻译： 一种从植物中产生干扰素诱导物的方法，包括用水从植物组织中提取水溶性干扰素诱导物，所述植物选自白术，金银花，车前子，紫草，Ligusticum，Cnidium，柴胡，Notopetellgium，Heracleum，Aralia， 番茄，Polygala，Sophora，Euchresta，黄芪，Sinomenium，Stephania，Cocculus，Cimicifuga，Rheum，Gastroida，芦笋，Pinellia，Evoida及其能够在提取混合物的环境温度至沸点温度下产生所述干扰素诱导剂的变异体 足以提取存在于植物组织中的所述干扰素诱导剂的主要部分的时间，形成上清液，分离上清液以产生含有上清液中所述干扰素诱导物主要部分的级分并从其中回收所述干扰素诱导剂。 优选地，分级可以通过超滤来实现。 由此获得的干扰素诱导物具有约30,000至约3,000,000的分子量，并且对于预防和治疗由病毒感染引起的人和动物的各种疾病具有潜在的兴趣。

公开(公告)号：US4871540A

公开(公告)日：1989-10-03

申请号：US887168

申请日：1986-07-17

申请人： Yasuhiko Kojima ,  Sadao Tamamura

发明人： Yasuhiko Kojima ,  Sadao Tamamura

IPC分类号： A61K38/00 ,  A61K31/715 ,  A61K36/00 ,  A61K36/899 ,  A61P37/00 ,  A61P43/00

CPC分类号： A61K31/715 ,  A61K36/899 ,  Y10S514/885

摘要： A biologically active substance comprising a kind of glycolipid having an elemental analysis (%) of H: 5.20-5.40, C: 42.55-44.17 and N: 3.94-4.08 and a molecular weight of about 30,000-10.sup.6 is obtained by extracting the tissue of a plant of maize (Zea mays Linne) or a variant thereof by extracting the tissue with water and recovering the desired active substance from the resultant extract. This substance is capable of inhibiting the formation of IgE antibodies and promoting the formation of IgG and IgM antibodies, and moreover exhibits various biological activities such as interferon-inducing activity, polyclonal B lymphocite activating activity, mitrogenic activity, adjuvant activity and anti-tumour activity. Thus, this substance is expected to be useful for preventing and treating, for example, aesthema, rhintitis, B-type hepatitis, AIDS and other allergic diseases and immunological insufficiency.

摘要翻译： 包含一种具有H：5.20-5.40，C：42.55-44.17和N：3.94-4.08的元素分析（％）和分子量约30,000-106的一种糖脂的生物活性物质是通过提取组织 玉米植物（Zea mays Linne）或其变体，通过用水提取组织并从所得提取物中回收所需的活性物质。 该物质能够抑制IgE抗体的形成并促进IgG和IgM抗体的形成，此外还表现出各种生物学活性，例如干扰素诱导活性，多克隆B淋巴细胞活化活性，有丝分裂活性，佐剂活性和抗肿瘤活性 。 因此，预期该物质可用于预防和治疗例如美学，鼻炎，B型肝炎，AIDS和其他过敏性疾病和免疫功能不全。