公开(公告)号：US20140088302A1

公开(公告)日：2014-03-27

申请号：US14113459

申请日：2012-04-25

申请人： Yasuhiro Nishitani ,  Toshiaki Aoki ,  Jun Sato ,  Kenji Yamawaki ,  Katsuki Yokoo ,  Masayuki SANO

发明人： Yasuhiro Nishitani ,  Toshiaki Aoki ,  Jun Sato ,  Kenji Yamawaki ,  Katsuki Yokoo ,  Masayuki SANO

IPC分类号： C07D501/60 ,  C07D519/00

CPC分类号： C07D501/60 ,  C07D501/18 ,  C07D501/46 ,  C07D519/00 ,  C07D519/06

摘要： The present invention provides a novel compound which has a wide antimicrobial spectrum, and in particular exhibits potent antimicrobial activity against beta-lactamase producing Gram negative bacteria. Specifically, the present invention provides a compound of the formula (I): wherein each symbol is as defined in the specification, or an amino-protected compound when the amino group is present on the ring in the 7-side chain, or a pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same.

摘要翻译： 本发明提供了具有广谱抗菌谱的新型化合物，并且特别表现出对产生β-内酰胺酶的革兰氏阴性菌具有强效的抗微生物活性。 具体而言，本发明提供了式（I）化合物：其中各符号如说明书中所定义，或氨基保护的化合物，当氨基存在于7-侧链的环上时，或药学上 可接受的盐，以及包含其的药物组合物。

公开(公告)号：US20120214240A1

公开(公告)日：2012-08-23

申请号：US13292953

申请日：2011-11-09

申请人： Mahito NAKANISHI ,  Ken NISHIMURA ,  Masayuki SANO ,  Manami OHTAKA

发明人： Mahito NAKANISHI ,  Ken NISHIMURA ,  Masayuki SANO ,  Manami OHTAKA

IPC分类号： C12N15/86 ,  C12N5/0789 ,  C07H21/02 ,  C12N7/01

CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/1131 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2760/18843

摘要： A reprogramming gene-loaded Sendai viral vector comprising Sendai virus genes and reprogramming genes, wherein the Sendai virus genes include an NP gene, P/C gene, M gene, F gene, HN gene and L gene, wherein each of the M gene, the F gene and the FIN gene is from a Sendai virus strain Cl.151-derived gene and wherein at least one of the M gene, the F gene and the HN gene is functionally deleted and the L gene encodes the amino-acid sequence of the L protein in which the amino-acid residue at position 1618 is valine and a method of producing the same.

摘要翻译： 包含仙台病毒基因和重编程基因的重编程基因载体仙台病毒载体，其中仙台病毒基因包括NP基因，P / C基因，M基因，F基因，HN基因和L基因，其中M基因， F基因和FIN基因来自仙台病毒株Cl.151衍生的基因，其中M基因，F基因和HN基因中的至少一个在功能上被缺失，并且L基因编码氨基酸序列 1618位氨基酸残基为缬氨酸的L蛋白及其制备方法。

公开(公告)号：US20100311171A1

公开(公告)日：2010-12-09

申请号：US12792580

申请日：2010-06-02

申请人： Mahito NAKANISHI ,  Ken NISHIMURA ,  Manami OHTAKA ,  Masayuki SANO

发明人： Mahito NAKANISHI ,  Ken NISHIMURA ,  Manami OHTAKA ,  Masayuki SANO

IPC分类号： C12N15/86 ,  C12N15/63 ,  C12N5/10 ,  C07H21/02 ,  C12N7/01

CPC分类号： C12N5/0696 ,  C12N15/1131 ,  C12N15/86 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2760/18843

摘要： Stem cell reprogramming genes cloned into a single sustained expression-type Sendai viral vector are shown to reprogram differentiated somatic cells into induced pluripotent stem (iPS) cells without integration of vector sequences into the host cell's genome. The genes are transduced into normal differentiated somatic cells via infection with recombinant Sendai virus. After expression of the reprogramming genes and subsequent induction of pluripotency, the vector genome RNA including the reprogramming genes is removed from the cell to establish an iPS cell that is genetically identical to the parent somatic differentiated cell thus reducing the risk of tumorigenic transformation caused by random integration of vector sequences into the host genome. The method promises to provide safe, autologous iPS cells that can be used for human cell replacement and regeneration therapeutic applications.

摘要翻译： 克隆到单一持续表达型仙台病毒载体中的干细胞重编程基因显示将分化的体细胞重编程为诱导多能干细胞（iPS）细胞，而不将载体序列整合到宿主细胞的基因组中。 通过感染重组仙台病毒将基因转导入正常分化的体细胞。 在重编程基因表达和随后的多能性诱导之后，从细胞中除去包括重编程基因的载体基因组RNA以建立与亲本体细胞分化细胞遗传相同的iPS细胞，从而降低由随机引起的致瘤转化的风险 将载体序列整合到宿主基因组中。 该方法有望提供可用于人类细胞替代和再生治疗应用的安全的自体iPS细胞。