公开(公告)号：US5012521A

公开(公告)日：1991-04-30

申请号：US291619

申请日：1988-12-29

申请人： Yasuo Endo ,  Shinji Akimichi ,  Tadayoshi Hirose ,  Motonori Furukawa ,  Kyoko Yoshida

发明人： Yasuo Endo ,  Shinji Akimichi ,  Tadayoshi Hirose ,  Motonori Furukawa ,  Kyoko Yoshida

IPC分类号： G06T11/80 ,  G06F3/033 ,  G06F3/048

CPC分类号： G06F3/04883 ,  G06F3/04845

摘要： When a pattern is manually drawn on a tablet with an electronic pen, a computer executes a graphic processing to display on a CRT a geometrically defined pattern which best resembles the manually drawn pattern. Moreover, a pattern element is erased, divided or copied by memorizing a graphic processing command in advance in a manner to correspond to a predetermined pattern or operator and by manually drawing this operator on the tablet with the electronic pen.The pattern data can be inputted to the computer to display the pattern by the same operations as those for drawing on a paper with writing apparatus so that the pattern can be drawn on the basis of the human engineering without feeling any physical disorder.

公开(公告)号：US20110231960A1

公开(公告)日：2011-09-22

申请号：US12990597

申请日：2009-04-28

申请人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui

发明人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui

IPC分类号： A01H5/00 ,  C12N15/63 ,  C07K19/00 ,  C07H21/04 ,  C12N5/10 ,  A01H5/10 ,  C07K7/08 ,  C07K14/00

CPC分类号： C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K2039/517 ,  C07K14/25 ,  C07K14/28 ,  C07K2319/00 ,  C07K2319/55 ,  C12N15/8258 ,  Y02A50/472 ,  Y02A50/474

摘要： A bacterial toxin protein such as a Shiga toxin protein is efficiently produced using plant cells. The plant cells are transformed using a DNA construct containing DNA encoding a hybrid protein in which the bacterial toxin proteins such as the Shiga toxin proteins are tandemly linked through a peptide having the following characteristics (A) and (B) to produce the bacterial toxin protein in the plant cells: (A) a number of amino acids is 12 to 30; and (B) a content of proline is 20 to 35%.

摘要翻译： 使用植物细胞有效地产生细菌毒素蛋白如志贺毒素蛋白质。 使用含有编码杂合蛋白的DNA的DNA构建体转化植物细胞，其中细菌毒素蛋白如志贺毒素蛋白通过具有以下特征（A）和（B）的肽串联连接以产生细菌毒素蛋白 在植物细胞中：（A）一些氨基酸为12〜30; （B）脯氨酸含量为20〜35％。

公开(公告)号：US20110002950A1

公开(公告)日：2011-01-06

申请号：US12667393

申请日：2008-03-25

申请人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui ,  Sou-ichi Makino ,  Keiko Kawamoto

发明人： Kazutoshi Sawada ,  Kazuya Yoshida ,  Mayumi Yoshida ,  Nobuo Yoshida ,  Kyoko Yoshida ,  Takeshi Matsui ,  Sou-ichi Makino ,  Keiko Kawamoto

IPC分类号： A61K39/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8258 ,  A61K39/0258 ,  A61K2039/517 ,  A61K2039/552 ,  C12N15/8257 ,  Y02A50/474

摘要： A technology for producing a pig edema disease vaccine at low cost and at high efficiency is developed. Specifically, a gene of a pig edema disease toxin protein (Stx2e protein) is efficiently expressed in plant cells to produce a plant vaccine for pig edema disease at low cost. An Stx2e protein including a secretory signal peptide derived from a plant added at an amino terminus is expressed in cells of a plant such as Lactuca sativa using the 5′-untranslated region of an alcohol dehydrogenase gene (ADH5′UTR) derived from a plant.

摘要翻译： 开发了一种以低成本，高效率生产猪水肿病疫苗的技术。 具体地说，猪水肿病毒素蛋白（Stx2e蛋白）的基因在植物细胞中有效表达，以低成本生产猪水肿病植物疫苗。 使用源于植物的醇脱氢酶基因（ADH5'UTR）的5'-非翻译区，在植物如莴苣植物的细胞中表达包含衍生自在氨基末端添加的植物的分泌信号肽的Stx2e蛋白质。