公开(公告)号：US20190195891A1

公开(公告)日：2019-06-27

申请号：US16325393

申请日：2017-08-18

Applicant: Yeda Research and Development Co. Ltd.

Inventor： David WALLACH ,  Andrew KOVALENKO ,  Seongmin YOON

IPC: G01N33/68 ,  C12Q1/48

CPC classification number: G01N33/6893 ,  A61K35/12 ,  A61K45/06 ,  C12Q1/485 ,  C12Q1/6883 ,  C12Q1/6888 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N2800/7095

Abstract: A method of detecting activation of a necroptosis activation pathway in a subject is disclosed. The method comprising: (a) obtaining a biological sample comprising exosomes from the subject; (b) detecting an activity or expression of a component of the necroptosis activation pathway in an exosome fraction of the biological sample, wherein an increase in the activity or expression of the component of the necroptosis activation pathway indicates the activation of said necroptosis activation pathway. Methods of diagnosing necroptosis or inflammation by determining the level of exosomes in a biological sample. Method of modulating endocytosis by inhibiting MLKL or a cell surface receptor and a pharmaceutical composition comprising a population of exosomes comprising a component of the necroptosis activation pathway and its use in therapy of diseases, such as inflammation and cancer.