公开(公告)号：US20190195891A1

公开(公告)日：2019-06-27

申请号：US16325393

申请日：2017-08-18

Applicant: Yeda Research and Development Co. Ltd.

Inventor： David WALLACH ,  Andrew KOVALENKO ,  Seongmin YOON

IPC: G01N33/68 ,  C12Q1/48

CPC classification number: G01N33/6893 ,  A61K35/12 ,  A61K45/06 ,  C12Q1/485 ,  C12Q1/6883 ,  C12Q1/6888 ,  C12Q2600/112 ,  C12Q2600/158 ,  G01N2800/7095

Abstract: A method of detecting activation of a necroptosis activation pathway in a subject is disclosed. The method comprising: (a) obtaining a biological sample comprising exosomes from the subject; (b) detecting an activity or expression of a component of the necroptosis activation pathway in an exosome fraction of the biological sample, wherein an increase in the activity or expression of the component of the necroptosis activation pathway indicates the activation of said necroptosis activation pathway. Methods of diagnosing necroptosis or inflammation by determining the level of exosomes in a biological sample. Method of modulating endocytosis by inhibiting MLKL or a cell surface receptor and a pharmaceutical composition comprising a population of exosomes comprising a component of the necroptosis activation pathway and its use in therapy of diseases, such as inflammation and cancer.

公开(公告)号：US20150329867A1

公开(公告)日：2015-11-19

申请号：US14791031

申请日：2015-07-02

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： David WALLACH ,  Andrei KOVALENKO ,  Tae-Bong KANG ,  Jin Chul KIM

IPC: C12N15/113 ,  C12Q1/68 ,  C12Q1/37 ,  G01N33/573

CPC classification number: C12N15/1137 ,  A61K38/1761 ,  A61K38/177 ,  A61K38/4873 ,  C12N15/1135 ,  C12N2310/14 ,  C12N2310/531 ,  C12Q1/37 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Y304/22061 ,  G01N33/573 ,  G01N2333/96466

Abstract: The invention relates to the treatment or prevention of an inflammatory skin disease, disorder or condition, by modulating a protein that is normally regulated by caspase-8 in the skin or by increasing caspase-8 activity or level in the skin. Another aspect of the invention relates to methods for diagnosing an inflammatory skin disease, disorder or condition or a predisposition to develop said disease disorder or condition in an individual. Further aspects of the invention relate to methods for identifying target proteins involved in the course or pathology of an inflammatory skin disease, disorder or condition and to methods of screening a candidate compound for treating said disease, disorder or condition. In particular, the invention relates to inflammatory skin diseases such as atopic dermatitis and psoriasis.

Abstract translation: 本发明涉及治疗或预防炎性皮肤疾病，病症或病症，通过调节通常由皮肤中的胱天蛋白酶-8调节的蛋白质或通过增加皮肤中的半胱天冬酶-8活性或水平。 本发明的另一方面涉及用于诊断炎症性皮肤疾病，病症或病症或倾向于在个体中发展所述疾病病症或病症的方法。 本发明的其它方面涉及用于鉴定涉及炎性皮肤病，病症或病症的病程或病理学的靶蛋白的方法以及筛选候选化合物以治疗所述疾病，病症或病症的方法。 特别地，本发明涉及炎性皮肤疾病如特应性皮炎和牛皮癣。