公开(公告)号：US11485971B2

公开(公告)日：2022-11-01

申请号：US16330774

申请日：2017-09-14

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido Amit ,  Diego Jaitin ,  David Lara-Astiaso ,  Assaf Weiner ,  Ido Yofe

IPC: C12N15/79 ,  C07H21/04 ,  C12N15/11 ,  C12N15/63 ,  C12N15/10 ,  C12N9/22 ,  C12Q1/6883 ,  C12N15/85 ,  C07H21/02

Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.