公开(公告)号：US11485971B2

公开(公告)日：2022-11-01

申请号：US16330774

申请日：2017-09-14

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido Amit ,  Diego Jaitin ,  David Lara-Astiaso ,  Assaf Weiner ,  Ido Yofe

IPC: C12N15/79 ,  C07H21/04 ,  C12N15/11 ,  C12N15/63 ,  C12N15/10 ,  C12N9/22 ,  C12Q1/6883 ,  C12N15/85 ,  C07H21/02

Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.

公开(公告)号：US20150307874A1

公开(公告)日：2015-10-29

申请号：US14795039

申请日：2015-07-09

Applicant: Yeda Research and Development Co. Ltd

Inventor： Diego JAITIN ,  Ido Amit ,  Hadas Keren-Shaul ,  Liran Valadarsky

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1065 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q2521/119 ,  C12Q2521/501 ,  C12Q2525/143 ,  C12Q2525/173 ,  C12Q2539/103 ,  C12Q2563/179

Abstract: Kits and methods for single cell or multiple cell transcriptome analysis are provided. An adapter polynucleotide is disclosed which comprises a double-stranded DNA portion of 15 base pairs and no more than 100 base pairs with a 3′ single stranded overhang of at least 3 bases and no more than 10 bases, wherein said double stranded DNA portion is at the 5′ end of the polynucleotide and wherein the sequence of said 3′ single stranded overhang is selected from the group consisting of SEQ ID NOs: 1-8 and 9, wherein the 5′ end of the strand of said double-stranded DNA which is devoid of said 3′ single stranded overhang comprises a free phosphate.

Abstract translation: 提供单细胞或多细胞转录组分析的试剂盒和方法。 公开了一种接头多核苷酸，其包含15个碱基对的双链DNA部分和不超过100个碱基对，其具有至少3个碱基和不超过10个碱基的3'单链突出端，其中所述双链DNA部分是 在多核苷酸的5'端，并且其中所述3'单链突出端的序列选自SEQ ID NO：1-8和9，其中所述双链DNA的链的5'末端 没有所述3'单链突出端包含游离磷酸盐。

公开(公告)号：US10590467B2

公开(公告)日：2020-03-17

申请号：US15303741

申请日：2015-04-16

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido Amit ,  David Lara-Astiaso ,  Meital Gury

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6816 ,  G01N33/53

Abstract: A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.

公开(公告)号：US20170137806A1

公开(公告)日：2017-05-18

申请号：US15412122

申请日：2017-01-23

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Diego JAITIN ,  Ido Amit ,  Hadas Keren-Shaul ,  Liran Valadarsky

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1065 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q2521/119 ,  C12Q2521/501 ,  C12Q2525/143 ,  C12Q2525/173 ,  C12Q2539/103 ,  C12Q2563/179

Abstract: Kits and methods for single cell or multiple cell transcriptome analysis are provided. An adapter polynucleotide is disclosed which comprises a double-stranded DNA portion of 15 base pairs and no more than 100 base pairs with a 3′single stranded overhang of at least 3 bases and no more than 10 bases, wherein said double stranded DNA portion is at the 5′ end of the polynucleotide and wherein the sequence of said 3′ single stranded overhang is selected from the group consisting of SEQ ID NOs: 1-8 and 9, wherein the 5′ end of the strand of said double-stranded DNA which is devoid of said 3′ single stranded overhang comprises a free phosphate.