公开(公告)号：US20220307008A1

公开(公告)日：2022-09-29

申请号：US17619722

申请日：2020-06-16

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido AMIT ,  Ido YOFE ,  Diego JAITIN ,  Yonatan KATZENELENBOGEN ,  Mami LENNIKOV ,  Assaf WEINER ,  Hadas KEREN-SHAUL

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N15/11

Abstract: A method of permeabilizing a cell is disclosed. Kits for permeabilizing cells and analysing RNA contents are also disclosed.

公开(公告)号：US20200263168A1

公开(公告)日：2020-08-20

申请号：US16784276

申请日：2020-02-07

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Diego JAITIN ,  Ido AMIT ,  Hadas KEREN-SHAUL ,  Liran VALADARSKY

IPC: C12N15/10 ,  C12Q1/6809 ,  C12Q1/6806

Abstract: Kits and methods for single cell or multiple cell transcriptome analysis are provided. An adapter polynucleotide is disclosed which comprises a double-stranded DNA portion of 15 base pairs and no more than 100 base pairs with a 3′single stranded overhang of at least 3 bases and no more than 10 bases, wherein the double stranded DNA portion is at the 5′ end of the polynucleotide and wherein the sequence of the 3′single stranded overhang is selected from the group consisting of SEQ ID NOs: 1-8 and 9, wherein the 5′ end of the strand of the double-stranded DNA which is devoid of the 3′single stranded overhang comprises a free phosphate.