公开(公告)号：US20220307008A1

公开(公告)日：2022-09-29

申请号：US17619722

申请日：2020-06-16

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido AMIT ,  Ido YOFE ,  Diego JAITIN ,  Yonatan KATZENELENBOGEN ,  Mami LENNIKOV ,  Assaf WEINER ,  Hadas KEREN-SHAUL

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N15/11

Abstract: A method of permeabilizing a cell is disclosed. Kits for permeabilizing cells and analysing RNA contents are also disclosed.

公开(公告)号：US20210293820A1

公开(公告)日：2021-09-23

申请号：US17334862

申请日：2021-05-31

Applicant: Yeda Research and Development Co. Ltd. ,  Stichting Het Nederlands Kanker Instituut - Antoni Van Leeuwenhoek Ziekenhuis

Inventor： Ido AMIT ,  Hanjie LI ,  Amos TANAY ,  Ido YOFE ,  Yaniv EYAL-LUBLING ,  Antonius Nicolaas Maria SCHUMACHER ,  Anne Magdalena VAN DER LEUN

IPC: G01N33/574 ,  C12N15/113 ,  G01N33/50

Abstract: Method of activating dysfunctional T cells, determining responsiveness of a subject having a tumor to an immune checkpoint inhibition are provided. Also provided is an agent capable of inhibiting a target gene or expression product thereof for use in treating a subject having a tumor. Additionally or alternatively provided is an immune checkpoint inhibitor and an agent capable of inhibiting a target gene or expression product thereof for use in treating a subject having a tumor.

公开(公告)号：US20190194653A1

公开(公告)日：2019-06-27

申请号：US16330774

申请日：2017-09-14

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ido AMIT ,  Diego JAITIN ,  David LARA-ASTIASO ,  Assaf WEINER ,  Ido YOFE

IPC: C12N15/11 ,  C12N15/10 ,  C12N9/22 ,  C12Q1/6883

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1093 ,  C12N15/63 ,  C12N2310/20 ,  C12N2800/80 ,  C12Q1/6883

Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.