公开(公告)号：US20110217239A1

公开(公告)日：2011-09-08

申请号：US12717143

申请日：2010-03-04

Applicant: Yi-Jang LEE ,  Cheng-Han TSAI ,  Liang-Ting LIN

Inventor： Yi-Jang LEE ,  Cheng-Han TSAI ,  Liang-Ting LIN

IPC: A61K49/00 ,  A61K31/7088 ,  C12N5/09 ,  C12N5/071 ,  A61P35/00

CPC classification number: A61K31/7088 ,  A61K49/00

Abstract: Disclosed herein are systems and methods for evaluating a protein of interest on growth inhibition of a tumor while following the tumor in vivo or in vitro through a co-expressed visible marker. The present disclosure provides an expression composition for stably transforming cells, preferably tumor cells. The expression composition includes two vectors respectively include polynucleotides encoding a protein of interest and a visible marker, wherein each polynucleotide is operably linked to a tet-on system such that the protein of interest and the visible marker are co-expressed upon activation of the tet-on system. The present disclosure also provides stably transfected cell lines, which may be used to access real-time biological processes, including tumor cell proliferation.

Abstract translation: 本文公开了用于在体内或体外通过共表达可见标记物追踪肿瘤同时评估肿瘤生长抑制的目的蛋白质的系统和方法。 本公开提供了用于稳定转化细胞，优选肿瘤细胞的表达组合物。 表达组合物包括两个载体，分别包括编码目的蛋白质和可见标记的多核苷酸，其中每个多核苷酸可操作地连接到tet-on系统，使得感兴趣的蛋白质和可见标记物在激活tet时共表达 - 系统。 本公开还提供了稳定转染的细胞系，其可用于接近实时生物过程，包括肿瘤细胞增殖。