摘要:
A method for producing firefly D-luciferin or a luminescent derivative thereof characterized by reacting a starting material for firefly L-luciferin in the presence of esterase, which contains at least one selected from the group consisting of (1) esters of firefly L-luciferin or derivatives thereof and (2) thioesters of firefly L-luciferin or derivatives thereof. The invention enables inexpensive determination of activity of firefly luciferase, long-time stable control of luminescence, and quantitative determination of L-cysteine, cystine and ATP.
摘要:
The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.
摘要:
The invention relates to a bioactive substance labeled with Cypridina luciferase and a quantum dot, the bioactive substance being at least one species selected from the group consisting of antibodies, peptides, organic compounds, hormones, enzyme substrates, sugar chains and nucleic acids.