Mutant luciferase
    1.
    发明授权
    Mutant luciferase 有权
    突变型萤光素酶

    公开(公告)号:US08147842B2

    公开(公告)日:2012-04-03

    申请号:US12304631

    申请日:2007-01-26

    IPC分类号: A61K39/00 C12N9/02 C07H21/04

    CPC分类号: C12N9/0069

    摘要: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.

    摘要翻译: 本发明的目的是提供具有改变的发射光谱的荧光素酶活性的突变荧光素酶。 将特定的氨基酸残基置于来自仙鹤草的萤光素酶中,然后筛选出具有与野生型萤光素酶的荧光素酶活性不同的荧光素酶活性的所得突变萤光素酶。

    Nucleic acid molecules encoding red and green emitting luciferases
    5.
    发明授权
    Nucleic acid molecules encoding red and green emitting luciferases 失效
    编码红色和绿色荧光素酶的核酸分子

    公开(公告)号:US06962986B2

    公开(公告)日:2005-11-08

    申请号:US09993874

    申请日:2001-11-14

    CPC分类号: C12N9/0069 C12Q1/66

    摘要: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.

    摘要翻译: 描述了编码荧光素酶的分离的核酸分子,其能够产生Phrixotrhix vivianii的绿色生物发光和Phrixothrix hirtus的红色生物发光。 由此编码的核酸分子和荧光素酶可用于诸如诊断方法和分子生物学工具的应用中。

    Two secretory luciferases
    6.
    发明授权
    Two secretory luciferases 有权
    两种分泌荧光素酶

    公开(公告)号:US08367357B2

    公开(公告)日:2013-02-05

    申请号:US12588671

    申请日:2009-10-23

    IPC分类号: C12Q1/66

    摘要: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.

    摘要翻译: 本发明是在不同启动子控制下的一种基因构建体或两种基因构建体或掺入Cypridina萤光素酶基因和桡足类荧光素酶的表达载体的组合。 这些基因构建体和表达载体可用于制备掺入Cypridina萤光素酶基因和桡足类荧光素酶的哺乳动物细胞,以能够在不同启动子的控制下稳定表达和细胞外分泌。