摘要:
An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
摘要:
A gene construct incorporating any of at least two luciferase genes which emit lights with different colors using an identical substrate such that the gene can be stably expressed in mammalian cells.
摘要:
A secretory or membrane-binding chimeric protein composed of an energy-generating protein and an energy-receiving protein linked one another wherein energy transfer can arise between the energy-generating protein and the energy-receiving protein.
摘要:
A monitor protein for measuring processing of a protein comprising a processing region which contains an amino acid residue or an amino acid residue sequence to be processed and a secretory chimeric protein which originates in the stereostructure of a protein containing at least a processing region formed by the processing and shows a change in luminescent and fluorescent properties.
摘要:
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
摘要:
The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
摘要:
The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.
摘要:
The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.
摘要:
A secretory or membrane-binding chimeric protein composed of an energy-generating protein and an energy-receiving protein linked one another wherein energy transfer can arise between the energy-generating protein and the energy-receiving protein.
摘要:
The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.