公开(公告)号：US20240361321A1

公开(公告)日：2024-10-31

申请号：US18685111

申请日：2022-08-23

申请人： INSTITUT PASTEUR ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

发明人： Thierry ROSE ,  Sophie GOYARD ,  Yves JANIN ,  Pierre LAFAYE ,  Gabriel AYME ,  Nicolas ESCRIOU ,  Marion GRANSAGNE

IPC分类号： G01N33/569 ,  C07K16/10 ,  C12Q1/66 ,  G01N33/68

CPC分类号： G01N33/56988 ,  C07K16/1003 ,  C07K16/1054 ,  C12Q1/66 ,  G01N33/6854 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/61 ,  G01N2333/161 ,  G01N2333/165 ,  G01N2333/90241

摘要： System for detecting an antigen comprising: —a first fusion protein with no luciferase activity comprising: —a N-terminal domain which comprises a first single domain antibody which is directed against a first epitope of said antigen and —a C-terminal domain which comprises a first fragment of a luciferase having the amino acid sequence SEQ ID NO: 1 or is a variant thereof and —a second fusion protein with no luciferase activity comprising: —a N-terminal domain which comprises a second single domain antibody which is directed against a second epitope of said antigen and —a C-terminal domain which comprises a second fragment of a luciferase having the amino acid sequence SEQ ID NO: 2 or is a variant thereof. Luminescence is emitted in the presence of a substrate when both the first fusion protein and the second fusion protein bind to said antigen.

公开(公告)号：US20240192196A1

公开(公告)日：2024-06-13

申请号：US18021348

申请日：2020-09-30

申请人： SUN YAT-SEN UNIVERSITY

发明人： Hui ZHANG ,  Yuewen LUO

IPC分类号： G01N33/50 ,  C07K14/005 ,  C12N15/52 ,  C12Q1/66

CPC分类号： G01N33/5023 ,  C07K14/005 ,  C12N15/52 ,  C12Q1/66 ,  C12N2770/20022 ,  C12N2770/20052 ,  G01N2333/90241 ,  G01N2500/10

摘要： The present disclosure discloses a novel coronavirus SARS-COV-2 safe replicon system and use thereof in screening an anti-SARS-COV-2 drug. The safe replicon system specifically comprises a nucleic acid sequence encoding a novel coronavirus SARS-COV-2 non-structural protein; and nucleic acid sequences of 5′ UTR and 3′ UTR of a novel coronavirus SARS-COV-2, a transcription regulatory region on which the novel coronavirus SARS-COV-2 non-structural protein can act, and a reporter gene. With the SARS-COV-2 safe replicon system, high-throughput screening of anti-SARS-COV-2 drugs and pharmacologic verification of drugs can be carried out independent of a biosafety level 3 laboratory, and the operation is simple and convenient.

公开(公告)号：US11781168B2

公开(公告)日：2023-10-10

申请号：US17240435

申请日：2021-04-26

申请人： PROMEGA CORPORATION

发明人： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC分类号： C07D487/04 ,  C12Q1/66 ,  C07D519/00 ,  A61K49/00 ,  G01N33/58 ,  H04N1/60

CPC分类号： C12Q1/66 ,  A61K49/0013 ,  C07D487/04 ,  C07D519/00 ,  G01N33/581 ,  H04N1/6041 ,  G01N2333/90241

摘要： Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I):




by reacting a compound of formula (xi):





with carbonyldiimidazole to form the compound of formula (I).

公开(公告)号：US09896712B2

公开(公告)日：2018-02-20

申请号：US14876671

申请日：2015-10-06

申请人： Colorado State University Research Foundation

发明人： Kenneth F. Reardon

IPC分类号： C12N9/02 ,  C12N11/00 ,  C12Q1/26 ,  C12Q1/34 ,  G01N21/77 ,  C12Q1/32 ,  G01N21/64 ,  C12M1/34 ,  C12M1/40

CPC分类号： C12Q1/26 ,  C12M1/34 ,  C12M1/40 ,  C12N9/0004 ,  C12N9/0069 ,  C12N9/0071 ,  C12Q1/32 ,  C12Y102/01002 ,  C12Y113/00 ,  C12Y114/00 ,  C12Y114/13 ,  G01N21/6408 ,  G01N21/6486 ,  G01N2021/6432 ,  G01N2333/90241 ,  G01N2333/90245

摘要： The present disclosure relates to biosensing systems and biosensing elements having increased storage capability and increased functional lifetimes through using compositions and methods for recycling cofactors.

公开(公告)号：US20170153228A1

公开(公告)日：2017-06-01

申请号：US15425923

申请日：2017-02-06

申请人： CITY OF HOPE

发明人： Markus KALKUM ,  Karine BAGRAMYAN

IPC分类号： G01N33/53 ,  G01N33/542

CPC分类号： G01N33/5308 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/569 ,  G01N33/573 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38 ,  G01N2333/90241

摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

公开(公告)号：US20170131275A1

公开(公告)日：2017-05-11

申请号：US15263619

申请日：2016-09-13

申请人： LABORATORY CORPORATION OF AMERICA HOLDINGS

发明人： DWIGHT LYMAN ANDERSON ,  ANDREW J. CONRAD ,  STEPHEN ERICKSON ,  JOSE S. GIL ,  BEN BARRETT HOPKINS

IPC分类号： G01N33/569 ,  G01N33/58 ,  G01N33/543

CPC分类号： G01N33/56911 ,  C12Q1/70 ,  G01N33/54326 ,  G01N33/569 ,  G01N33/581 ,  G01N2333/90241 ,  G01N2469/10

摘要： Disclosed are methods and systems for the isolation and detection of microbes from a sample. The use of binding agents for isolation of a microbe of interest from a sample are described. In certain embodiments, the methods use ribosome-based and/or bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. For example, embodiments of the present invention can achieve total amplification of at least 10,000 from a single infected cell.

公开(公告)号：US20170038367A1

公开(公告)日：2017-02-09

申请号：US15107266

申请日：2014-12-23

申请人： UNIVERSITÉ DE MONTRÉAL

发明人： DAVID COTNOIR-WHITE ,  SYLVIE MADER ,  ÉTIENNE GAGNON ,  MICHEL BOUVIER

IPC分类号： G01N33/542 ,  G01N33/68 ,  G01N33/74

CPC分类号： G01N33/542 ,  G01N33/6845 ,  G01N33/743 ,  G01N2333/705 ,  G01N2333/70567 ,  G01N2333/726 ,  G01N2333/90241 ,  G01N2500/04

摘要： Described herein is a bioluminescence resonance energy transfer (BRET) based system and method to monitor ternary complex formation in real-time in live cells with high sensitivity and accuracy. This system transfers energy simultaneously between a luciferase donor and intermediate and terminal acceptors, appropriately chosen to also enable transfer from the intermediate to terminal acceptor while minimizing contaminating signals. The system may also be adapted for quaternary complex detection by including a protein complementation assay (PCA) component. The system is broadly applicable to the detection of any protein ternary/quaternary complex such as those involving nuclear receptors, GPCRs, Receptor Tyrosine Kinase (RTKs), multimeric enzymes or structural proteins.

摘要翻译： 本文描述了一种基于生物发光共振能量转移（BRET）的系统和方法，以高灵敏度和准确度实时监测活细胞中的三元复合物形成。 该系统在萤光素酶供体和中间体和末端受体之间同时转移能量，适当选择以使得能够从中间体转移到末端受体，同时最小化污染信号。 该系统还可以通过包括蛋白质互补测定（PCA）组分来适应四级复合物检测。 该系统广泛适用于任何蛋白质三元/季复合物的检测，例如涉及核受体，GPCR，受体酪氨酸激酶（RTK），多聚体酶或结构蛋白的蛋白。

公开(公告)号：US09476870B2

公开(公告)日：2016-10-25

申请号：US14719500

申请日：2015-05-22

申请人： PTC Therapeutics, Inc.

发明人： Liangxian Cao ,  Nikolai Naryshkin ,  Charles Romfo ,  Matthew C. Pellegrini ,  Anuradha Roy ,  Panayiota Trifillis ,  Christopher R. Trotta

IPC分类号： G01N33/50 ,  C12Q1/68 ,  G01N33/74

CPC分类号： G01N33/5023 ,  C12Q1/6897 ,  G01N33/74 ,  G01N2333/49 ,  G01N2333/90241

摘要： The invention relates to the fields of screening assays, compounds, and methods for altering gene expression and protein levels. In particular, the invention includes assays to screen for agents capable of modulating gene expression in a UTR-dependent manner and agents capable of modulating gene expression.

公开(公告)号：US09464368B2

公开(公告)日：2016-10-11

申请号：US12918372

申请日：2009-02-12

申请人： Eyal Zussman ,  Yael Dror ,  Jonathan Charles Kuhn

发明人： Eyal Zussman ,  Yael Dror ,  Jonathan Charles Kuhn

IPC分类号： D01D5/24 ,  D01F1/08 ,  A61K9/00 ,  D01D5/00 ,  B82Y30/00 ,  C02F3/10 ,  C02F3/34 ,  D01D5/247 ,  D01F1/10 ,  D01F8/14 ,  G01N33/543 ,  C02F101/18 ,  C02F101/20 ,  C02F101/30 ,  C02F101/36

CPC分类号： C12N11/04 ,  A61K9/0092 ,  A61K38/44 ,  B82Y30/00 ,  C02F3/102 ,  C02F3/34 ,  C02F3/342 ,  C02F2101/18 ,  C02F2101/20 ,  C02F2101/306 ,  C02F2101/36 ,  C02F2305/08 ,  C09D167/04 ,  C09D171/02 ,  C12M21/18 ,  C12M23/06 ,  C12M23/16 ,  C12Q1/66 ,  C12Y113/12007 ,  C12Y114/16001 ,  D01D5/003 ,  D01D5/247 ,  D01F1/10 ,  D01F8/14 ,  G01N33/54393 ,  G01N2333/90241 ,  Y02W10/15 ,  Y10T428/1393

摘要： Provided is a method of attaching a molecule-of-interest to a microtube, by co-electrospinning two polymeric solutions through co-axial capillaries, wherein a first polymeric solution of the two polymeric solutions is for forming a shell of the microtube and a second polymeric solution of the two polymeric solutions is for forming a coat over an internal surface of the shell, the first polymeric solution is selected solidifying faster than the second polymeric solution and a solvent of the second polymeric solution is selected incapable of dissolving the first polymeric solution and wherein the second polymeric solution comprises the molecule-of-interest, thereby attaching the molecule-of-interest to the microtube. Also provided is an electrospun microtube comprising an electrospun shell, an electrospun coat over an internal surface of the shell and a molecule-of-interest attached to the microtube.

摘要翻译： 提供了通过共同静电纺丝两个聚合物溶液通过同轴毛细管将感兴趣分子附着到微管的方法，其中两个聚合物溶液的第一聚合溶液用于形成微管的壳，第二聚合溶液用于形成微管 两种聚合物溶液的聚合物溶液用于在壳的内表面上形成涂层，选择第一聚合物溶液比第二聚合物溶液更快地固化，并且选择第二聚合物溶液的溶剂不能溶解第一聚合物溶液 并且其中所述第二聚合物溶液包含感兴趣的分子，从而将感兴趣的分子附着到微管。 还提供了一种电纺微管，其包括电纺壳，在壳的内表面上的电纺丝涂层和附着于微管的感兴趣分子。

公开(公告)号：US20160289732A1

公开(公告)日：2016-10-06

申请号：US15150676

申请日：2016-05-10

申请人： 3M INNOVATIVE PROPERTIES COMPANY

发明人： G. MARCO BOMMARITO ,  MANJIRI T. KSHIRSAGAR ,  TING LIU ,  TIMOTHY J. NIES ,  SWARNALATHA SWAMINATHAN

IPC分类号： C12Q1/66 ,  A61L2/28 ,  C12Q1/22

CPC分类号： C12Q1/66 ,  A61B90/70 ,  A61B2090/702 ,  A61L2/28 ,  C12Q1/22 ,  C12Y113/12007 ,  G01N2333/90241 ,  Y10T436/13

摘要： The present disclosure provides a monitoring device comprising a test composition, a test element comprising a test portion to which the test composition is releasably adhered, a detection reagent, and a container comprising a first end with an opening and a second end opposite the first end. The test composition comprises a predetermined quantity of tracer analyte. The container is configured to receive the test portion and configured to be operationally coupled to an analytical instrument. The tracer analyte and the detection reagent each are capable of participating in one or more chemical reaction that results in the formation of a detectable product. A method of using the monitoring device to assess the efficacy of a washing process is also provided.