公开(公告)号：US07341864B2

公开(公告)日：2008-03-11

申请号：US10182262

申请日：2001-01-29

申请人： Yoshitaka Sakamoto ,  Masafumi Koide ,  Juichiro Nakashima ,  Susumu Satoh ,  Sousuke Miyoshi ,  Akiko Suzuki ,  Hiroyuki Arakawa ,  Hiroshi Marusawa

发明人： Yoshitaka Sakamoto ,  Masafumi Koide ,  Juichiro Nakashima ,  Susumu Satoh ,  Sousuke Miyoshi ,  Akiko Suzuki ,  Hiroyuki Arakawa ,  Hiroshi Marusawa

IPC分类号： C12M1/42 ,  C12M3/00 ,  C12N13/00 ,  C12N15/87 ,  C12N15/82

CPC分类号： C12N15/87 ,  A61K48/00 ,  A61K48/0083 ,  C12M35/02

摘要： A molecule introducing apparatus is provided with a power supply circuit 20 for supplying power, a step-up transformer 30 which is supplied with power from the power supply circuit 20 and which outputs a high voltage, a switching transistor 22 that blocks off and allows the supply of power from the power supply circuit 20 to the step-up transformer 30 and which generates an instantaneous high voltage, and a pair of electrode probes 50 for applying the instantaneous high voltage generated at the step-up transformer 30 to a predetermined region in a body. The molecule introducing apparatus oscillates DNA arranged outside a cell by the instantaneous high voltage so as to introduce the DNA into the cell.

摘要翻译： 分子引入装置设置有用于供电的电源电路20，从电源电路20提供电力并输出高电压的升压变压器30，截止的开关晶体管22， 从电源电路20向升压变压器30供电并产生瞬时高电压;以及一对电极探针50，用于将升压变压器30产生的瞬时高压施加到预定区域 身体。 分子导入装置通过瞬时高电压振荡排列在细胞外的DNA，以将DNA引入细胞。

公开(公告)号：US07402435B2

公开(公告)日：2008-07-22

申请号：US10470732

申请日：2002-02-08

申请人： Sousuke Miyoshi ,  Akiko Ohkubo ,  Noriyuki Morikawa ,  Yasuhiro Ogawa ,  Shintaro Nishimura ,  Masao Fukagawa ,  Hiroyuki Arakawa ,  Jyunko Zenkou ,  Susumu Sato

发明人： Sousuke Miyoshi ,  Akiko Ohkubo ,  Noriyuki Morikawa ,  Yasuhiro Ogawa ,  Shintaro Nishimura ,  Masao Fukagawa ,  Hiroyuki Arakawa ,  Jyunko Zenkou ,  Susumu Sato

IPC分类号： C12N15/00 ,  C12N15/02

CPC分类号： C12N15/87 ,  C12M35/02 ,  C12N15/02

摘要： A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.

摘要翻译： 将各种选择的分子高效转移到各种细胞中的方法和融合细胞的方法。 用冷气体等离子体处理细胞和/或选择的分子，例如多核苷酸，从而将位于细胞周围的选定分子转移到细胞中，或者通过用冷气体等离子体处理细胞来融合细胞。 此外，提供了一种用于转移所选分子或具有用于将选定分子转移到细胞中的具有冷气体等离子体产生单元的融合电池的装置。

公开(公告)号：US06582905B1

公开(公告)日：2003-06-24

申请号：US09230378

申请日：1999-03-15

申请人： Hiromitsu Nakauchi ,  Yukio Nakamura ,  Sousuke Miyoshi ,  Dong ku Kim

发明人： Hiromitsu Nakauchi ,  Yukio Nakamura ,  Sousuke Miyoshi ,  Dong ku Kim

IPC分类号： C12Q168

CPC分类号： C12N15/1051 ,  C07K14/705

摘要： The present invention provides a method of cloning a protein coding for a membrane protein without the need of using anyantibody or ligand. The method of cloning a membrane protein comprises selecting a protein having a signal sequence, using a DNA coding for the signal sequence and N-terminal sequence of the protein as the reporter gene, and screening for a gene for a protein having a transmembrane domain(s). The present invention is concerned also with the CDNA obtained by using such method, a vector containing the DNA, a transformant as transformed with the vector, and the transcription product of the DNA as produced by the transformant.

摘要翻译： 本发明提供了克隆编码膜蛋白质的蛋白质的方法，而不需要使用任何抗体或配体。克隆膜蛋白的方法包括：使用编码信号序列的DNA选择具有信号序列的蛋白质，将N 作为报告基因的蛋白质的末端序列，筛选具有跨膜结构域的蛋白质的基因。本发明还涉及通过使用该方法获得的CDNA，含有DNA的载体，转化体 用载体转化，转化体产生的DNA的转录产物。