公开(公告)号：US08377676B2

公开(公告)日：2013-02-19

申请号：US12810902

申请日：2008-12-23

申请人： Young-Doug Sohn ,  Bum Joon Kim ,  Ok Hwan Kim ,  Kyoung Jun Kim ,  Ji Hun Shin ,  Sung Yu Hong ,  Jae Hoon Hwang ,  Kwang Hoe Chung

发明人： Young-Doug Sohn ,  Bum Joon Kim ,  Ok Hwan Kim ,  Kyoung Jun Kim ,  Ji Hun Shin ,  Sung Yu Hong ,  Jae Hoon Hwang ,  Kwang Hoe Chung

IPC分类号： C12N9/74 ,  C12N15/57

CPC分类号： C12N9/6418 ,  C07K2319/02

摘要： The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.

摘要翻译： 本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型α因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高出约20倍的产量获得重组巴曲酶。 此外，使用本发明的序列制备的重组巴曲酶与天然存在的巴曲酶相比具有显着似乎合理的活性和稳定性。

公开(公告)号：US20110136207A1

公开(公告)日：2011-06-09

申请号：US12810902

申请日：2008-12-23

申请人： Young-Doug Sohn ,  Bum Joon Kim ,  Ok Hwan Kim ,  Kyoung Jun Kim ,  Ji Hun Shin ,  Sung Yu Hong ,  Jae Hoon Hwang ,  Kwang Hoe Chung

发明人： Young-Doug Sohn ,  Bum Joon Kim ,  Ok Hwan Kim ,  Kyoung Jun Kim ,  Ji Hun Shin ,  Sung Yu Hong ,  Jae Hoon Hwang ,  Kwang Hoe Chung

IPC分类号： C12N9/48 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N9/6418 ,  C07K2319/02

摘要： The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.

摘要翻译： 本发明涉及一种编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列，以及使用其的载体和转化体。 本发明的巴曲酶编码核苷酸序列在酵母，特别是巴斯德毕赤酵母中表现出优异的表达效率，并且以比天然存在的巴曲酶编码序列高出4-13倍的产量获得重组巴曲酶。 使用巴曲酶编码核苷酸序列以及本发明的突变型α因子分泌信号肽序列的蛋白质表达系统以比天然存在的巴曲酶编码序列高出约20倍的产量获得重组巴曲酶。 此外，使用本发明的序列制备的重组巴曲酶与天然存在的巴曲酶相比具有显着似乎合理的活性和稳定性。

公开(公告)号：US20050032189A1

公开(公告)日：2005-02-10

申请号：US10089473

申请日：2002-03-26

申请人： Kwang-Hoe Chung ,  Doo-Sik Kim ,  Sung-Yu Hong ,  You-Seok Koh ,  Young-Doug Sohn ,  Weon-Kyoo You ,  Yang-Soo Jang ,  Chin-Kyu Huh

发明人： Kwang-Hoe Chung ,  Doo-Sik Kim ,  Sung-Yu Hong ,  You-Seok Koh ,  Young-Doug Sohn ,  Weon-Kyoo You ,  Yang-Soo Jang ,  Chin-Kyu Huh

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P7/02 ,  A61P35/00 ,  C07K1/16 ,  C07K14/46 ,  C07K14/745 ,  C12N1/19 ,  C12P21/02 ,  C12R1/84 ,  C12N9/64 ,  A61K38/46 ,  C07H21/04 ,  C12N1/18 ,  C12N15/74

CPC分类号： C07K14/745 ,  A61K38/00 ,  C07K14/46

摘要： The present invention relates to Saxatilin, a protein derived from the venom of a Korean snake, Agkistrodon saxatilis emelianov, a process for preparing Saxatilin, and pharmaceutical application of the same as anti-platelet aggregation agent and anti-tumor agent. The present inventors purified Saxatilin from the venom of Agkistrodon saxatilis emelianov, cloned its cDNA, and prepared recombinant Saxatilin by culturing a microorganism transformed with an expression vector containing the cDNA. Saxatilin effectively suppresses the platelet aggregation and angiogenesis of tumor cells, which makes possible its practical application as an active ingredient of anti-platelet agent and anti-tumor agent.

摘要翻译： 本发明涉及萨克西林，一种衍生自韩国蛇的毒液，猕猴桃，猕猴桃，一种制备萨克西林的方法，以及其抗血小板聚集剂和抗肿瘤剂的药物应用。 本发明人从猕猴桃痘病毒的毒液中纯化了萨克西林，克隆了其cDNA，并通过培养用含有cDNA的表达载体转化的微生物来制备重组萨克斯汀。 萨克西汀有效抑制肿瘤细胞的血小板聚集和血管生成，使其作为抗血小板剂和抗肿瘤剂的有效成分成为可能。

公开(公告)号：US07033788B2

公开(公告)日：2006-04-25

申请号：US10089473

申请日：2002-03-26

申请人： Kwang-Hoe Chung ,  Doo-sik Kim ,  Sung-Yu Hong ,  You-Seok Koh ,  Young-Doug Sohn ,  Weon-Kyoo You ,  Yang-Soo Jang ,  Chin-Kyu Huh

发明人： Kwang-Hoe Chung ,  Doo-sik Kim ,  Sung-Yu Hong ,  You-Seok Koh ,  Young-Doug Sohn ,  Weon-Kyoo You ,  Yang-Soo Jang ,  Chin-Kyu Huh

IPC分类号： C12N15/12 ,  C12N15/63 ,  C12N1/19 ,  C12N1/00 ,  A61K38/17

CPC分类号： C07K14/745 ,  A61K38/00 ,  C07K14/46

摘要： The present invention relates to Saxatilin, a protein derived from the venom of a Korean snake, Agkistrodon saxatilis emelianov, a process for preparing Saxatilin, and pharmaceutical application of the same as anti-platelet aggregation agent and anti-tumor agent. The present inventors purified Saxatilin from the venom of Agkistrodon saxatilis emelianov, cloned its cDNA, and prepared recombinant Saxatilin by culturing a microorganism transformed with an expression vector containing the cDNA. Saxatilin effectively suppresses the platelet aggregation and angiogenesis of tumor cells, which makes possible its practical application as an active ingredient of anti-platelet agent and anti-tumor agent.

摘要翻译： 本发明涉及萨克西林，一种衍生自韩国蛇的毒液，猕猴桃，猕猴桃，一种制备萨克西林的方法，以及其抗血小板聚集剂和抗肿瘤剂的药物应用。 本发明人从猕猴桃痘病毒的毒液中纯化了萨克西林，克隆了其cDNA，并通过培养用含有cDNA的表达载体转化的微生物来制备重组萨克斯汀。 萨克西汀有效抑制肿瘤细胞的血小板聚集和血管生成，使其作为抗血小板剂和抗肿瘤剂的有效成分成为可能。