公开(公告)号：US07148011B2

公开(公告)日：2006-12-12

申请号：US10380254

申请日：2001-09-21

申请人： Yuji Sugita ,  Ryoichi Hashida ,  Kaoru Ogawa ,  Tomoko Fujishima ,  Takeshi Nagasu ,  Gozoh Tsujimoto ,  Eiki Takahashi

发明人： Yuji Sugita ,  Ryoichi Hashida ,  Kaoru Ogawa ,  Tomoko Fujishima ,  Takeshi Nagasu ,  Gozoh Tsujimoto ,  Eiki Takahashi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  C07K14/47 ,  C12Q2600/158

摘要： The differential display method was used to search for a gene whose expression level in eosinophils collected from patients with atopic dermatitis differs in the exacerbation stage and in the remission stage. As a result, gene “2090-05” showing a significant increase in expression in eosinophils of patients in the remission stage was isolated. This gene is usable in testing for an allergic disease and screening for a candidate compound for a therapeutic agent therefor an allergic disease.

摘要翻译： 差异显示法用于寻找在特应性皮炎患者中收集的嗜酸性粒细胞的表达水平在恶化阶段和缓解期不同的基因。 结果，分离出在缓解期患者嗜酸性粒细胞中表达显着增加的基因“2090-05”。 该基因可用于测试过敏性疾病并筛选用于治疗剂的候选化合物用于过敏性疾病。

公开(公告)号：US06986990B1

公开(公告)日：2006-01-17

申请号：US10019832

申请日：2000-04-26

申请人： Takeshi Nagasu ,  Yuji Sugita ,  Tomoko Fujishima ,  Tadahiro Oshida ,  Masaya Obayashi ,  Shigemichi Gunji ,  Izumi Obayashi ,  Yukiho Imai ,  Nei Yoshida ,  Kaoru Ogawa ,  Keiko Matsui

发明人： Takeshi Nagasu ,  Yuji Sugita ,  Tomoko Fujishima ,  Tadahiro Oshida ,  Masaya Obayashi ,  Shigemichi Gunji ,  Izumi Obayashi ,  Yukiho Imai ,  Nei Yoshida ,  Kaoru Ogawa ,  Keiko Matsui

IPC分类号： C12Q1/66

CPC分类号： C07K14/47

摘要： T cells from multiple subjects who showed different cedar pollen specific IgE titers were prepared before and after cedar pollen dispersal, and differential display analysis was performed to screen for pollen antigen responsive genes. As a result, a novel gene was successfully isolated whose expression is significantly higher in subjects with high cedar pollen specific IgE titer. The inventors have found that it is possible to use this gene to test for an allergic disease and to screen for candidate compounds for therapeutic drugs for an allergic disease.

摘要翻译： 在雪松花粉散布前后制备了显示不同雪松花粉特异性IgE滴度的多个受试者的T细胞，进行差异显示分析筛选花粉抗原应答基因。 结果，成功地分离出一种新型基因，其表达在具有高雪松花粉特异性IgE滴度的受试者中显着更高。 发明人已经发现，可以使用该基因来测试过敏性疾病并筛选用于过敏性疾病的治疗药物的候选化合物。