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    Method and apparatus for measuring hematological sample
    5.
    发明授权
    Method and apparatus for measuring hematological sample 有权
    用于测量血液样品的方法和装置

    公开(公告)号：US07892841B2

    公开(公告)日：2011-02-22

    申请号：US11727806

    申请日：2007-03-28

    申请人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    发明人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    IPC分类号： G01N33/48 ,  G01N21/47 ,  G01N21/64

    CPC分类号： G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

    摘要： A measuring method and a measuring apparatus which can classify and count myeloblast more precisely without influence of other component in a sample including platelet aggregation in measurement of a blood sample with a flowcytometry, wherein damage is given to a cell membrane of erythrocyte and mature leukocyte contained in a hematological sample, a hemocyte in which a cell membrane is damaged is constricted, and this is dyeing-treated with a fluorescent dye which can stain a nucleic acid to obtain a sample, the sample is measured with a flowcytometer, and a cell contained in a first cell group containing myeloblast, which is specified based on forward scattered light information and side scattered light information, and contained in a second cell group containing myeloblast, which is specified based on forward scattered light information and fluorescent information, is counted as myeloblast.

    摘要翻译： 一种测量方法和测量装置，可以更精确地对成像细胞进行分类和计数，而不影响样品中的其他成分，包括用流式细胞术测量血液样品中的血小板聚集，其中包含红细胞和成熟白细胞的细胞膜受损 在血液样品中，细胞膜损伤的血细胞被收缩，并用荧光染料进行染色处理，荧光染料可以染色核酸以获得样品，用流式细胞仪测量样品，并且含有 在基于前向散射光信息和侧向散射光信息指定的包含成髓细胞的第一细胞组中，包含在基于前向散射光信息和荧光信息指定的含有成髓细胞的第二细胞群中，被计为成髓细胞 。

    Reagent for immature leukocyte analysis and reagent kit
    7.
    发明授权
    Reagent for immature leukocyte analysis and reagent kit 有权
    未成熟白细胞分析试剂和试剂盒

    公开(公告)号：US07625757B2

    公开(公告)日：2009-12-01

    申请号：US11650962

    申请日：2007-01-09

    申请人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    发明人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    IPC分类号： G01N31/00 ,  G01N33/48

    CPC分类号： G01N33/5094 ,  G01N15/1468 ,  G01N2015/008 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125

    摘要： A reagent for analyzing immature leukocyte capable of analyzing accurately immature leukocyte such as myeloblast and immature granulocyte, and mature leukocyte, which reagent has broader allowable range of sample processing conditions than conventional ones, and a reagent kit are provided. The present invention relates to a reagent for analyzing immature leukocyte containing a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte in the sample, a solubilizing agent for causing contraction to damaged blood cell, sugar, a dye for staining nucleic acid.

    摘要翻译： 提供能够分析准确地未成熟白细胞例如成髓细胞和未成熟粒细胞的未成熟白细胞和成熟白细胞的试剂，该试剂比常规试剂处理条件更宽的允许范围和试剂盒。 本发明涉及一种用于分析含有表面活性剂的未成熟白细胞用于对样品中红细胞和成熟白细胞的细胞膜造成损伤的试剂，用于引起血细胞损伤的增溶剂，糖，用于染色核酸的染料 。

    Method and apparatus for measuring hematological sample
    10.
    发明申请
    Method and apparatus for measuring hematological sample 有权
    用于测量血液样品的方法和装置

    公开(公告)号：US20070231913A1

    公开(公告)日：2007-10-04

    申请号：US11727806

    申请日：2007-03-28

    申请人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    发明人： Tomohiro Tsuji ,  Ayumu Yoshida ,  Shinichiro Oguni

    IPC分类号： G01N33/48

    CPC分类号： G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1477 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

    摘要： A measuring method and a measuring apparatus which can classify and count myeloblast more precisely without influence of other component in a sample including platelet aggregation in measurement of a blood sample with a flowcytometry, wherein damage is given to a cell membrane of erythrocyte and mature leukocyte contained in a hematological sample, a hemocyte in which a cell membrane is damaged is constricted, and this is dyeing-treated with a fluorescent dye which can stain a nucleic acid to obtain a sample, the sample is measured with a flowcytometer, and a cell contained in a first cell group containing myeloblast, which is specified based on forward scattered light information and side scattered light information, and contained in a second cell group containing myeloblast, which is specified based on forward scattered light information and fluorescent information, is counted as myeloblast.

    摘要翻译： 一种测量方法和测量装置，可以更精确地对成像细胞进行分类和计数，而不影响样品中的其他成分，包括用流式细胞术测量血液样品中的血小板聚集，其中包含红细胞和成熟白细胞的细胞膜受损 在血液样品中，细胞膜损伤的血细胞被收缩，并用荧光染料进行染色处理，荧光染料可以染色核酸以获得样品，用流式细胞仪测量样品，并且含有 在基于前向散射光信息和侧向散射光信息指定的包含成髓细胞的第一细胞组中，包含在基于前向散射光信息和荧光信息指定的含有成髓细胞的第二细胞群中，被计为成髓细胞 。