公开(公告)号：US09402358B2

公开(公告)日：2016-08-02

申请号：US13390969

申请日：2010-08-18

申请人： Yunxing C. Cui ,  Jill R. Bryan ,  Donald G. Maum ,  Gregory J. Gilles ,  Terry R. Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan J. VanOpdorp ,  Tina M. Kaiser ,  Ning Zhou

发明人： Yunxing C. Cui ,  Jill R. Bryan ,  Donald G. Maum ,  Gregory J. Gilles ,  Terry R. Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan J. VanOpdorp ,  Tina M. Kaiser ,  Ning Zhou

IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12N5/00 ,  A01H5/10 ,  C12N9/02 ,  C12N15/82

CPC分类号： A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8213 ,  C12N15/8274 ,  C12Q1/6895 ,  C12Q2600/16 ,  C12Y113/00

摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

摘要翻译： 本发明部分涉及植物育种和除草剂耐受性植物。 本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。 另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。 测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。 还提供了用于进行测定的试剂盒和条件。

公开(公告)号：US20120222153A1

公开(公告)日：2012-08-30

申请号：US13390969

申请日：2010-08-18

申请人： Yunxing C. Cui ,  Jill R. Bryan ,  Donald G. Maum ,  Gregory J. Gilles ,  Terry R. Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan J. VanOpdorp ,  Tina M. Kaiser ,  Ning Zhou

发明人： Yunxing C. Cui ,  Jill R. Bryan ,  Donald G. Maum ,  Gregory J. Gilles ,  Terry R. Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan J. VanOpdorp ,  Tina M. Kaiser ,  Ning Zhou

IPC分类号： A01H5/00 ,  C12N15/82 ,  A01H5/02 ,  A01H1/02 ,  A01H5/06 ,  A01H5/12 ,  C12N15/29 ,  C12Q1/68 ,  A01H5/10 ,  A01H5/04

CPC分类号： A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8213 ,  C12N15/8274 ,  C12Q1/6895 ,  C12Q2600/16 ,  C12Y113/00

摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

摘要翻译： 本发明部分涉及植物育种和除草剂耐受性植物。 本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。 另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。 测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。 还提供了用于进行测定的试剂盒和条件。

公开(公告)号：US09944944B2

公开(公告)日：2018-04-17

申请号：US13511990

申请日：2010-11-24

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

IPC分类号： C12N15/82 ,  A01H1/02 ,  A01H5/10 ,  A01H1/04 ,  C12N9/02 ,  C12Q1/68

CPC分类号： C12N15/8274 ,  A01H1/02 ,  A01H1/04 ,  A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12Q1/6895 ,  C12Q2600/156

摘要： This invention includes a novel aad-12 transformation event for herbicide tolerance in soybean plants—referred to herein as pDAB4468-0416. This invention includes a heterologous polynucleotide inserted into a specific site within the genome of a soybean cell. In some embodiments, said event/polynucleotide can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (a soybean, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US09738904B2

公开(公告)日：2017-08-22

申请号：US14234923

申请日：2012-07-26

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Dawn M. Parkhurst ,  Ning Zhou ,  Barry Wiggins ,  Dayakar Pareddy ,  Gregory A. Bradfisch ,  James E. Dripps ,  Sandra G. Toledo ,  Nathan Bard ,  Michael Vercauteren ,  Nandi Nagaraj ,  Brandon Bishop ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Dawn M. Parkhurst ,  Ning Zhou ,  Barry Wiggins ,  Dayakar Pareddy ,  Gregory A. Bradfisch ,  James E. Dripps ,  Sandra G. Toledo ,  Nathan Bard ,  Michael Vercauteren ,  Nandi Nagaraj ,  Brandon Bishop ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N5/04 ,  C07H21/04 ,  C12N15/82 ,  C12Q1/68 ,  A01N37/38 ,  A01N37/44 ,  A01N65/20

CPC分类号： C12N15/8286 ,  A01N37/38 ,  A01N37/44 ,  A01N65/20 ,  C12N15/8209 ,  C12N15/8274 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/162

摘要： A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1::pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1::pDAB4468.04.16.1 comprises genes encoding AAD-12, Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

公开(公告)号：US20140344994A1

公开(公告)日：2014-11-20

申请号：US14234923

申请日：2012-07-26

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Dawn M. Parkhurst ,  Ning Zhou ,  Barry Wiggins ,  Dayakar Pareddy ,  Gregory A. Bradfisch ,  James E. Dripps ,  Sandra G. Toledo ,  Nathan Bard ,  Michael Vercauteren ,  Nandi Nagaraj ,  Brandon Bishop ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Dawn M. Parkhurst ,  Ning Zhou ,  Barry Wiggins ,  Dayakar Pareddy ,  Gregory A. Bradfisch ,  James E. Dripps ,  Sandra G. Toledo ,  Nathan Bard ,  Michael Vercauteren ,  Nandi Nagaraj ,  Brandon Bishop ,  Gregory J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barnes ,  Nathan J. VanOpdorp ,  Yonghe Bai

IPC分类号： C12N15/82 ,  A01N65/20 ,  A01N37/38 ,  A01N37/44

CPC分类号： C12N15/8286 ,  A01N37/38 ,  A01N37/44 ,  A01N65/20 ,  C12N15/8209 ,  C12N15/8274 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/162

摘要： A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1 comprises genes encoding AAD-12, CrylF, CrylAc (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

摘要翻译： 大豆事件9582.814.19.1和大豆事件pDAB4468.04.16.1的育种堆栈导致了大豆事件pDAB9582.814.19.1 :: pDAB4468.04.16.1。 大豆事件pDAB9582.814.19.1 :: pDAB4468.04.16.1包含编码AAD-12，Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及有效的作物保护方法 并保护储存的产品。

公开(公告)号：US08785728B2

公开(公告)日：2014-07-22

申请号：US13226789

申请日：2011-09-07

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Dawn M. Parkhurst ,  Julissa Colon ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Dawn M. Parkhurst ,  Julissa Colon ,  Yonghe Bai

IPC分类号： C12N15/82 ,  A01H1/00 ,  C07H21/04

CPC分类号： C12N15/8274 ,  A01H1/04 ,  C12N9/0004

摘要： This invention relates to soybean event pDAB4472-1606 (Event 1606). This invention includes a novel aad-12 transformation event in soybean plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a soybean cell. This invention also relates in part to plant breeding and herbicide tolerant plants. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins.

摘要翻译： 本发明涉及大豆事件pDAB4472-1606（事件1606）。 本发明包括大豆植物中的新型aad-12转化事件，其包含插入大豆细胞基因组内的特定位点的本文所述的多核苷酸序列。 本发明还部分涉及植物育种和除草剂耐受性植物。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。

公开(公告)号：US20120110688A1

公开(公告)日：2012-05-03

申请号：US13226789

申请日：2011-09-07

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Dawn M. Parkhurst ,  Julissa Colon ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Gregory J. Gilles ,  Terry R. Wright ,  Dawn M. Parkhurst ,  Julissa Colon ,  Yonghe Bai

IPC分类号： A01H1/02 ,  C12N5/10 ,  A01H5/10 ,  C12N15/82 ,  A01H5/02 ,  A01H5/06 ,  A01H5/12 ,  C07H21/04 ,  C12N15/53 ,  A01N39/04 ,  A01N57/20 ,  A01P13/00 ,  A01N25/00 ,  A01C7/00 ,  A01H5/00

CPC分类号： C12N15/8274 ,  A01H1/04 ,  C12N9/0004

摘要： This invention relates to soybean event pDAB4472-1606 (Event 1606). This invention includes a novel aad-12 transformation event in soybean plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a soybean cell. This invention also relates in part to plant breeding and herbicide tolerant plants. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins.

摘要翻译： 本发明涉及大豆事件pDAB4472-1606（事件1606）。 本发明包括大豆植物中的新型aad-12转化事件，其包含插入大豆细胞基因组内的特定位点的本文所述的多核苷酸序列。 本发明还部分涉及植物育种和除草剂耐受性植物。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。

公开(公告)号：US20110289620A1

公开(公告)日：2011-11-24

申请号：US13032142

申请日：2011-02-22

申请人： Yunxing Cui ,  Jill Rene Bryan ,  Donald Glynn Maum ,  Terry Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan Vanopdorp ,  Tina Marie Kaiser ,  Ning Zhou ,  Steve Allan McMaster ,  Mark Allen Peterson ,  Leon Bo Braxton ,  Gregory J. Gilles

发明人： Yunxing Cui ,  Jill Rene Bryan ,  Donald Glynn Maum ,  Terry Wright ,  Jennifer L. Hamilton ,  Nicole L. Arnold ,  Nathan Vanopdorp ,  Tina Marie Kaiser ,  Ning Zhou ,  Steve Allan McMaster ,  Mark Allen Peterson ,  Leon Bo Braxton ,  Gregory J. Gilles

IPC分类号： A01H5/00 ,  C12N15/82 ,  A01H5/02 ,  A01H5/04 ,  A01H5/06 ,  A01H5/12 ,  C12N15/29 ,  C12Q1/68 ,  C07H21/04 ,  A01H1/02 ,  A01N37/14 ,  A01N57/20 ,  A01P13/00 ,  G01N21/76 ,  A01G1/00 ,  A01C7/00 ,  A01H5/10

CPC分类号： A01N37/38 ,  A01H5/10 ,  A01N57/20 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8274

摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided. The subject invention also includes related methods of controlling weeds.

摘要翻译： 本发明部分涉及植物育种和除草剂耐受性植物。 本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。 另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。 测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。 还提供了用于进行测定的试剂盒和条件。 本发明还包括控制杂草的相关方法。

公开(公告)号：US08598413B2

公开(公告)日：2013-12-03

申请号：US13032142

申请日：2011-02-22

申请人： Yunxing Cui ,  Jill Rene Bryan ,  Donald Glynn Maum ,  Terry Wright ,  Jennifer L. Hamilton ,  Nicole L Arnold ,  Nathan VanOpdorp ,  Tina Marie Kaiser ,  Ning Zhou ,  Steve Allan McMaster ,  Mark Allen Peterson ,  Leon Bo Braxton ,  Gregory J. Gilles

发明人： Yunxing Cui ,  Jill Rene Bryan ,  Donald Glynn Maum ,  Terry Wright ,  Jennifer L. Hamilton ,  Nicole L Arnold ,  Nathan VanOpdorp ,  Tina Marie Kaiser ,  Ning Zhou ,  Steve Allan McMaster ,  Mark Allen Peterson ,  Leon Bo Braxton ,  Gregory J. Gilles

IPC分类号： C12N15/82 ,  A01H5/10 ,  A01H1/00

CPC分类号： A01N37/38 ,  A01H5/10 ,  A01N57/20 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8274

摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided. The subject invention also includes related methods of controlling weeds.

摘要翻译： 本发明部分涉及植物育种和除草剂耐受性植物。 本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。 在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。 另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。 测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。 还提供了用于进行测定的试剂盒和条件。 本发明还包括控制杂草的相关方法。

公开(公告)号：US20130324408A1

公开(公告)日：2013-12-05

申请号：US13511990

申请日：2010-11-24

申请人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Greg J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barns ,  Nathan J. VanOpdrop ,  Yonghe Bai

发明人： Yunxing C. Cui ,  Thomas Hoffman ,  Ning Zhou ,  Greg J. Gilles ,  Terry R. Wright ,  Julissa Colon ,  Ricardo A. Barns ,  Nathan J. VanOpdrop ,  Yonghe Bai

IPC分类号： C12N15/82 ,  C12Q1/68 ,  C12N9/02

CPC分类号： C12N15/8274 ,  A01H1/02 ,  A01H1/04 ,  A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12Q1/6895 ,  C12Q2600/156

摘要： This invention includes a novel aad-12 transformation event for herbicide tolerance in soybean plants—referred to herein as pDAB4468-0416. This invention includes a heterologous polynucleotide inserted into a specific site within the genome of a soybean cell. In some embodiments, said event/polynucleotide can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (a soybean, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

摘要翻译： 本发明包括大豆植物中除草剂耐受性的新型aad-12转化事件 - 本文称为pDAB4468-0416。 本发明包括插入大豆细胞基因组内特定位点的异源多核苷酸。 在一些实施方案中，所述事件/多核苷酸可以与其它性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制性蛋白质。 另外，本发明提供了用于检测样品（例如大豆）中受试者事件的存在的测定法。 测定可以基于重组构建体的DNA序列，插入大豆基因组中，以及插入位点侧翼的基因组序列。 还提供了用于进行测定的试剂盒和条件。