公开(公告)号：US4743544A

公开(公告)日：1988-05-10

申请号：US609530

申请日：1984-05-14

申请人： Yuzaburo Namba ,  Tohru Naraki ,  Takashi Sawada ,  Toyohiro Kitamura ,  Minoru Tohda ,  Tomiaki Morimoto

发明人： Yuzaburo Namba ,  Tohru Naraki ,  Takashi Sawada ,  Toyohiro Kitamura ,  Minoru Tohda ,  Tomiaki Morimoto

IPC分类号： G01N33/543 ,  B01L3/00 ,  G01N33/53 ,  G01N33/566

CPC分类号： B01L3/50851 ,  G01N33/53

摘要： Improved method for the measurement of substance comprising simultaneously measuring a plurality of specimens of a substance to be measured by enzyme immunoassay. The process comprises:a. reacting specimens and a material which will be specifically combined with the substance to be measured and which has been made in the solid phase;b. separating the reaction mixture in step (a) into the solid phase and the liquid phase;c. reacting the solid phase with an enzyme-labelled product of a material which will be specifically combined with the substance to be measured;d. separating the reaction mixture in the step (c) into the solid phase and the liquid phase;e. reacting the solid phase or the liquid phase with a substrate for the enzyme in the step (c); andf. measuring the optical density of the reaction mixture. The improvement comprises, in step (a), successively contacting the respective specimens with the material which will be specifically combined with the substance to be measured and which has been made in the solid phase, under cooling condition at a temperature ranging from 0.degree. C. to 15.degree. C., followed by simultaneously incubating them at a temperature ranging from room temperature to 45.degree. C. The fluctuation of results can be remarkably smaller by the employment of cooling means.

摘要翻译： 用于测量物质的改进方法包括通过酶免疫测定同时测量待测物质的多个样品。 该过程包括：a。 反应样品和将与待测物质特异性结合并在固相中制备的材料; b。 将步骤（a）中的反应混合物分离成固相和液相; C。 使固相与待测物质特异性组合的材料的酶标记产物反应; d。 将步骤（c）中的反应混合物分离成固相和液相; e。 在步骤（c）中使固相或液相与酶的底物反应; 和f。 测量反应混合物的光密度。 改进包括在步骤（a）中，在冷却条件下，在0℃的温度下，将相应的试样与待测物质特异性结合的材料相继接触，并在固相中制成 至15℃，然后在室温至45℃的温度下同时孵育。通过使用冷却装置，结果的波动可以显着更小。