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公开(公告)号：US20170204199A1
公开(公告)日：2017-07-20
申请号：US15314496
申请日：2015-05-29
申请人： ZYMEWORKS INC.
发明人： Mario Sanches , Thomas Spreter Von Kreudenstein , Dunja Urosev , Stacey A. L. Tom-Yew , Adam Louis Corper , Igor Edmondo Paolo D'Angelo , Yang-Chieh Chou , Surjit Bhimarao Dixit
CPC分类号： C07K16/468 , C07K16/2863 , C07K16/32 , C07K16/36 , C07K2317/21 , C07K2317/24 , C07K2317/31 , C07K2317/51 , C07K2317/52 , C07K2317/522 , C07K2317/55 , C07K2317/56 , C07K2317/92 , C07K2317/94
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the CH1 and/or CL domains, one or more amino acid modifications in the VH and/or VL domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
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公开(公告)号：US20150211001A1
公开(公告)日：2015-07-30
申请号：US14432153
申请日：2013-10-03
申请人： Jason BAARDSNES , Maureen O'CONNOR-MCCOURT , ZYMEWORKS INC. , NATIONAL RESEARCH COUNCIL CANADA (NRC)
发明人： Anders Ohrn , Adam Louis Corper , Gordon Yiu Kon Ng , Antonios Samiotakis , Yang-Chieh Chou , Jason Baardsnes , Maureen O'Connor-McCourt
CPC分类号： C12N15/1055 , A61K39/00 , C07K16/468 , C07K2317/55 , G01N21/6486
摘要： Provided is a method of quantitatively determining the ability of individual IgG heavy chains to selectively pair with a particular IgG light chain when the heavy chains and two unique light chains are co-expressed. The method provides results with reasonable throughput and is robust and accurate. The co-expressed heavy and light chains do not need to be isolated and purified which enables more efficient screening.
摘要翻译： 提供了当重链和两个独特的轻链共表达时，定量测定个体IgG重链与特定IgG轻链选择性配对的能力的方法。 该方法提供了具有合理吞吐量的结果，并且是鲁棒和准确的。 共表达的重链和轻链不需要被分离和纯化，这使得能够更有效地筛选。
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公开(公告)号：US11286293B2
公开(公告)日：2022-03-29
申请号：US16122417
申请日：2018-09-05
申请人： ZYMEWORKS INC.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A. L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario , Mario Sanches
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the CH1 and/or CL domains, one or more amino acid modifications in the VH and/or VL domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
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4.发明申请公开(公告)号：US20150307594A1
公开(公告)日：2015-10-29
申请号：US14648222
申请日：2013-11-28
申请人： ZYMEWORKS INC.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A.L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario
CPC分类号： C07K16/00 , C07K16/2863 , C07K16/32 , C07K16/36 , C07K2317/10 , C07K2317/31 , C07K2317/35 , C07K2317/51 , C07K2317/515 , C07K2317/52 , C07K2317/522 , C07K2317/524 , C07K2317/526 , C07K2317/53 , C07K2317/55 , C07K2317/72 , C07K2317/92 , C07K2317/94 , G06F19/24
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the C H and/or C L domains, one or more amino acid modifications in the V H and/or V L domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
摘要翻译： 本发明提供了可以包含第一异二聚体和第二异二聚体的异二聚体对，其中每个异二聚体包含免疫球蛋白重链或其片段和免疫球蛋白轻链或其片段。 至少一个异源二聚体可以包含C H和/或C L结构域中的一个或多个氨基酸修饰，V H和/或V L结构域中的一个或多个氨基酸修饰或其组合。 修饰的氨基酸可以是轻链和重链之间的界面的一部分，并且通常被修饰以在每个重链和期望的轻链之间产生优先配对，使得当两条重链和两条轻链 异源二聚体对在细胞中共表达，第一异源二聚体的重链优先与轻链之一而不是另一个重合。 同样，第二异二聚体的重链通常优先与第二轻链配对，而不是首先。
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公开(公告)号：US20220251242A1
公开(公告)日：2022-08-11
申请号：US17652557
申请日：2022-02-25
申请人： ZYMEWORKS INC.
发明人： Mario SANCHES , Thomas Spreter Von Kreudenstein , Dunja Urosev , Stacey A.L. Tom-Yew , Adam Louis Corper , Igor Edmondo Paolo D'Angelo , Yang-Chieh Chou , Surjit Bhimarao Dixit
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the CH1 and/or CL domains, one or more amino acid modifications in the VH and/or VL domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
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公开(公告)号：US09914785B2
公开(公告)日：2018-03-13
申请号：US14092804
申请日：2013-11-27
申请人： Zymeworks Inc.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A. L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario , Mario Sanches
CPC分类号： C07K16/36 , C07K2317/55 , C07K2317/94
摘要： The present invention provides heterodimer pairs comprising a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain. At least one of the heterodimers comprises amino acid modifications in the CH1 and/or CL domains, amino acid modifications in the VH and/or VL domains or a combination thereof. The modified amino acid residues are part of the interface between the light chain and heavy chain and are modified in order to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a mammalian cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer preferentially pairs with the second light chain rather than first.
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公开(公告)号：US11306156B2
公开(公告)日：2022-04-19
申请号：US15314496
申请日：2015-05-29
申请人： ZYMEWORKS INC.
发明人： Mario Sanches , Thomas Spreter Von Kreudenstein , Dunja Urosev , Stacey A. L. Tom-Yew , Adam Louis Corper , Igor Edmondo Paolo D'Angelo , Yang-Chieh Chou , Surjit Bhimarao Dixit
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the CH1 and/or CL domains, one or more amino acid modifications in the VH and/or VL domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
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公开(公告)号：US20210309760A1
公开(公告)日：2021-10-07
申请号：US17343198
申请日：2021-06-09
申请人： Zymeworks Inc.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A.L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario , Mario Sanches
IPC分类号： C07K16/36
摘要： Provided are heterodimer pairs comprising a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain. At least one of the heterodimers comprises amino acid modifications in the CH1 and/or CL domains, amino acid modifications in the VH and/or VL domains or a combination thereof. The modified amino acid residues are part of the interface between the light chain and heavy chain and are modified in order to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a mammalian cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer preferentially pairs with the second light chain rather than first.
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公开(公告)号：US11078296B2
公开(公告)日：2021-08-03
申请号：US15896170
申请日：2018-02-14
申请人： Zymeworks Inc.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A. L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario , Mario Sanches
摘要： Provided are heterodimer pairs comprising a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain. At least one of the heterodimers comprises amino acid modifications in the CH1 and/or CL domains, amino acid modifications in the VH and/or VL domains or a combination thereof. The modified amino acid residues are part of the interface between the light chain and heavy chain and are modified in order to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a mammalian cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer preferentially pairs with the second light chain rather than first.
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公开(公告)号：US10077298B2
公开(公告)日：2018-09-18
申请号：US14648222
申请日：2013-11-28
申请人： Zymeworks Inc.
发明人： Adam Louis Corper , Dunja Urosev , Stacey A. L. Tom-Yew , Dustin Weyland Blue Bleile , Thomas Spreter Von Kreudenstein , Surjit Dixit , Paula Irene Lario , Mario Sanches
CPC分类号： C07K16/00 , C07K16/2863 , C07K16/32 , C07K16/36 , C07K2317/10 , C07K2317/31 , C07K2317/35 , C07K2317/51 , C07K2317/515 , C07K2317/52 , C07K2317/522 , C07K2317/524 , C07K2317/526 , C07K2317/53 , C07K2317/55 , C07K2317/72 , C07K2317/92 , C07K2317/94 , G16B40/00
摘要： The present invention provides heterodimer pairs that can comprise a first heterodimer and a second heterodimer wherein each heterodimer comprises an immunoglobulin heavy chain or fragment thereof and an immunoglobulin light chain or fragment thereof. At least one of the heterodimers can comprise one or more amino acid modifications in the CH1 and/or CL domains, one or more amino acid modifications in the VH and/or VL domains, or a combination thereof. The modified amino acid(s) can be part of the interface between the light chain and heavy chain and are typically modified to create preferential pairing between each heavy chain and a desired light chain such that when the two heavy chains and two light chains of the heterodimer pair are co-expressed in a cell, the heavy chain of the first heterodimer preferentially pairs with one of the light chains rather than the other. Likewise, the heavy chain of the second heterodimer typically preferentially pairs with the second light chain rather than first.
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