公开(公告)号：US20170137895A1

公开(公告)日：2017-05-18

申请号：US15311006

申请日：2015-05-15

申请人： ZYMONOSTICS APS

发明人： Magnus Stougaard ,  Birgitta Ruth Knudsen ,  Marianne Smedegaard Hede ,  Jonas Thomsen ,  Yi-Ping Ho

IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12Q1/533

CPC分类号： C12Q1/6893 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/25 ,  C12Q1/485 ,  C12Q1/533 ,  C12Q1/68 ,  C12Q1/689 ,  G01N33/573 ,  G01N2333/9015 ,  G01N2333/91245 ,  G01N2800/26 ,  Y02A50/58 ,  C12Q2521/501 ,  C12Q2533/107 ,  C12Q2565/629 ,  C12Q2521/507 ,  C12Q2521/519

摘要： The invention provides a method of identifying a microorganism that expresses a nucleic acid-modifying enzyme, in sample, the method comprising: (a) contacting a nucleic acid substrate targeted by the nucleic acid-modifying enzyme with the sample; (b) adding a further nucleic acid molecule to the sample which nucleic acid molecule is ligated to the nucleic acid substrate in the presence of the nucleic acid-modifying enzyme to form a ligation product which comprises a linear single strand of nucleic acid that is capable of being detected; and (c) detecting the presence of the ligation product.