公开(公告)号：US11920175B2

公开(公告)日：2024-03-05

申请号：US17259161

申请日：2019-07-09

申请人： Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory

发明人： Xinde Xu ,  Tian Xie ,  Shengfan Wang ,  Qiuyan Wang ,  Jianyong Zheng ,  Zhaowu Zeng ,  Xiaopu Yin ,  Xuejun Lao ,  Kangzhong Shao

IPC分类号： C07C403/24 ,  C12P23/00

CPC分类号： C12P23/00 ,  C07C403/24 ,  C07B2200/13 ,  C07C2601/16

摘要： A method for extracting and isolating a lutein crystal from a vegetable oil resin containing a lutein diester, comprises: dissolving lipase into deionized water to form an enzyme solution; dissolving a lutein extract into an alcohol solvent containing the deionized water to form a uniform alcohol solution; adding the enzyme solution to the alcohol solution for performing hydrolysis, and stirring same to obtain a lutein solution; filtering and performing filtration isolation on the lutein solution to obtain a crystalline; re-dissolving the crystalline into a non-polar organic solvent, and using deionized water for washing a water-soluble impurity; recycling and cooling the organic solvent to obtain a recrystallization; and isolating and drying the recrystallization to obtain the lutein crystal. In this method, selectivity is strong, reaction time is short, no waste water is produced, process is environment-friendly and suitable for industrial production, and obtained lutein crystal is high in purity and yield.