公开(公告)号：US20230183662A1

公开(公告)日：2023-06-15

申请号：US17552076

申请日：2021-12-15

Applicant: Zhejiang University

Inventor： Yuejin Hua ,  Shengjie Li ,  Liangyan Wang ,  Jianling Cai ,  Xiaoting Hua

IPC: C12N9/10

CPC classification number: C12N9/1007 ,  C12Y201/01037

Abstract: A DNA methyltransferase is derived from Deinococcus radiodurans and has a typical conservative structural domain of DNA methyltransferase. The DNA methyltransferase includes: an AdoMet binding region containing a “FxGxG” conservative sequence, a target sequence recognition region and a catalytic region containing a “TSPPY” conservative sequence sequentially from N-terminal to C-terminal; and belongs to α-type DNA methyltransferase category. The recognized substrate DNA conservative sequence is 5′-CCGCGG-3′, a methylation modified position is N4 site of second cytosine to generate a 4mC type modified base, and an optimum temperature for methylated reaction is in a range of 25-37° C. The DNA methyltransferase can specific-recognize the conservative motif of “CCGCGG” and methylate the N4 site of the second cytosine to produce the 4mC modified base, which is a N4-Cytosine DNA methyltransferase.