公开(公告)号：US07592508B1

公开(公告)日：2009-09-22

申请号：US10009590

申请日：1999-06-11

申请人： Zhi Xian Chen ,  Lian Hui Zhang

发明人： Zhi Xian Chen ,  Lian Hui Zhang

IPC分类号： C12N15/82

CPC分类号： C12N15/8209 ,  C12N15/8205

摘要： A method is disclosed for producing a transgenic cotton plant by Agrobacterium-mediated transformation of petiole tissue. The method comprises the steps of (a) obtaining cotton petiole explants, (b) exposing the petiole explants to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the petiole explant, (c) culturing the petiole explants to induce callus formation, (d) selecting transformed callus that expresses the exogenous gene, (e) culturing the selected callus in suspension culture to induce formation of embryoids, (f) regenerating the embryoids into whole transgenic cotton plants.

摘要翻译： 公开了通过土壤杆菌介导的叶柄组织转化来生产转基因棉花植物的方法。 该方法包括以下步骤：（a）获得棉花外植体，（b）将叶柄外植体暴露于含有包含外源基因和选择性标记的载体的根癌土壤杆菌培养物中，所述农杆菌能够实现稳定转移 （e）培养叶柄外植体以诱导愈伤组织形成，（d）选择表达外源基因的转化愈伤组织，（e）培养所选择的愈伤组织 在悬浮培养物中诱导胚状体的形成，（f）将胚状体再生成整个转基因棉花植物。

公开(公告)号：US20080182105A1

公开(公告)日：2008-07-31

申请号：US11791142

申请日：2005-11-09

申请人： Lian Hui Wang ,  Ji-En Wu ,  Lian Hui Zhang

发明人： Lian Hui Wang ,  Ji-En Wu ,  Lian Hui Zhang

IPC分类号： B32B33/00 ,  H01L21/44

CPC分类号： B82Y30/00 ,  C30B7/00 ,  C30B29/605 ,  Y10T428/2991

摘要： The present invention relates to a method of forming a core/shell nanocrystal of semiconductor material. Typically the core may comprise CdTe and the shell may be CdS. The shell is synthesised on the core in an aqueous solution. In the method, the previously synthesised cores are placed in the aqueous solution, reactants that form the shell and a thiol such as 3-mercaptopropionic acid (MPA) are added, and the mixture is refluxed until the completion of the shell at the desired thickness. The synthesis of the shell is aided by the provision of an interface zone between the shell and core so that lattice mismatch between the core and shell is reduced. The interface zone may be produced using a method that provides a gradient alloyed core with increased levels of sulphur, for example, at the surface relative to the centre of the core. Alternatively the interface zone may be a separate layer on a homogenous core.

摘要翻译： 本发明涉及形成半导体材料的核/壳纳米晶体的方法。 通常，芯可以包含CdTe，壳可以是CdS。 壳体在水溶液中在芯上合成。 在该方法中，将先前合成的核心置于水溶液中，加入形成壳的反应物和诸如3-巯基丙酸（MPA）的硫醇，并将混合物回流至完成壳体至所需厚度 。 通过在壳和芯之间提供界面区域来帮助壳的合成，使得芯和壳之间的晶格失配减小。 界面区可以使用提供梯度合金化铁芯与硫含量增加的方法来制备，例如在相对于芯的中心的表面处。 或者，界面区可以是均匀芯上的单独层。

公开(公告)号：US07235712B1

公开(公告)日：2007-06-26

申请号：US10203963

申请日：2000-02-15

申请人： Lian Hui Zhang ,  Xianzhen Li ,  Daohai Zhang

发明人： Lian Hui Zhang ,  Xianzhen Li ,  Daohai Zhang

IPC分类号： C12N15/31 ,  C12N15/82 ,  C12N15/61 ,  C12N15/62 ,  A01H5/00

CPC分类号： C12N9/90 ,  C12P19/12 ,  C12P19/24 ,  C12R1/22 ,  C12Y504/99011

摘要： Two strains of a novel bacterial species, Klebsiella singaporensis LX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity.

摘要翻译： 要求两种新型细菌物种，克莱伯氏菌（Klebsiella singaporensis）LX3和LX21。 还要求编码编码新型异麦芽酮糖合酶KIS的核苷酸序列（kis）。 还要求保护的是在植物中生产异麦芽酮糖的方法，该方法包括将这种核酸序列导入该植物的细胞中，所述核酸序列编码以允许所述细胞表达所述核酸序列的方式将蔗糖转化成异麦芽酮糖的酶， 以及分离编码KIS蛋白的核苷酸序列的功能性克隆方法，其包括以下步骤：（a）从供体生物体制备含有编码在合适宿主生物体中的异麦芽酮糖生物合成活性的DNA序列的基因库，（b） 通过其增加的还原糖含量筛选基因库中感兴趣的克隆，和（c）分离含有编码具有异麦芽酮糖生物合成活性的蛋白质的DNA的克隆。

公开(公告)号：US07098014B2

公开(公告)日：2006-08-29

申请号：US10502351

申请日：2002-01-23

申请人： Lian Hui Zhang ,  Jin Ling Xu ,  Yi Han Lin

发明人： Lian Hui Zhang ,  Jin Ling Xu ,  Yi Han Lin

IPC分类号： C12N9/14

CPC分类号： C12N9/18 ,  A01K2217/05 ,  C12N15/8281

摘要： This invention provides a gene, qsbA, which encodes a protein useful for inactivating certain bacterial quorum-sensing signal molecules (N-acyl homoserine lactones) which participate in bacterial virulence and biofilm differentiation pathways. This gene was isolated from Ralstonia sp., strain XJ12B. The invention also provides the QsbA protein, which possesses N-acyl homoserine lactone inactivating activity.

摘要翻译： 本发明提供了一种编码可用于灭活参与细菌毒力和生物膜分化途径的某些细菌群体感应信号分子（N-酰基高丝氨酸内酯）的蛋白质的qsbA基因。 从Ralstonia sp。，菌株XJ12B分离该基因。 本发明还提供具有N-酰基高丝氨酸内酯失活活性的QsbA蛋白。