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    Analysis of gene expression profiles using sequential hybridization
    1.
    发明申请
    Analysis of gene expression profiles using sequential hybridization 审中-公开
    使用顺序杂交分析基因表达谱

    公开(公告)号：US20070148690A1

    公开(公告)日：2007-06-28

    申请号：US11703921

    申请日：2007-02-06

    申请人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

    发明人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6876 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/519 ,  C12Q2537/155 ,  C12Q2537/149

    摘要： This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.

    摘要翻译： 本发明一般涉及基因表达谱的分析。 特别地，本发明提供了一种分析细胞基因表达谱的方法，该方法包括：a）从细胞提供分离的mRNA或cDNA靶序列; b）将所述分离的mRNA或cDNA靶序列与多个核苷酸探针顺序杂交; 和c）评估所述分离的mRNA或cDNA靶序列与所述多个核苷酸探针之间的顺序杂交，以分析所述细胞的基因表达谱。 还提供了分析基因表达谱的系统。 还提供用于检测杂交信号的光学装置。