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    Analysis of gene expression profiles using sequential hybridization
    1.
    发明申请
    Analysis of gene expression profiles using sequential hybridization 审中-公开
    使用顺序杂交分析基因表达谱

    公开(公告)号：US20070148690A1

    公开(公告)日：2007-06-28

    申请号：US11703921

    申请日：2007-02-06

    申请人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

    发明人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6876 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/519 ,  C12Q2537/155 ,  C12Q2537/149

    摘要： This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.

    摘要翻译： 本发明一般涉及基因表达谱的分析。 特别地，本发明提供了一种分析细胞基因表达谱的方法，该方法包括：a）从细胞提供分离的mRNA或cDNA靶序列; b）将所述分离的mRNA或cDNA靶序列与多个核苷酸探针顺序杂交; 和c）评估所述分离的mRNA或cDNA靶序列与所述多个核苷酸探针之间的顺序杂交，以分析所述细胞的基因表达谱。 还提供了分析基因表达谱的系统。 还提供用于检测杂交信号的光学装置。

    DNA sequencing method and system
    3.
    发明授权
    DNA sequencing method and system 有权
    DNA测序方法和系统

    公开(公告)号：US08481266B2

    公开(公告)日：2013-07-09

    申请号：US12742995

    申请日：2008-11-17

    申请人： Zhifeng Shao ,  Sitong Sheng

    发明人： Zhifeng Shao ,  Sitong Sheng

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6874 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/501 ,  C12Q2565/531

    摘要： The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.

    摘要翻译： 本发明涉及基因工程领域，提供了DNA测序方法和系统。 所述DNA测序方法包括以下步骤：A.将所述DNA加工成多个DNA片段，然后构建成多个DNA标签; B.扩增每个单个DNA标签，然后加工成单链DNA; C.利用可连接DNA标签的锚，并具有至少一个退化碱基来对每一个DNA标签进行排序，从而产生测序信号; D.通过测序信号获得每个DNA片段的序列。 所述DNA测序系统包括：短标签构建单位，放大单位，测序反应单位和信号处理单位。 在本发明中，由于通过使用具有至少一个退化性的测序锚来对DNA标签进行测序，所以扩展了可直接测序的DNA标签的长度。 短和长DNA标签都可以测序。 因此扩展了DNA测序的应用。

    DNA SEQUENCING METHOD AND SYSTEM
    4.
    发明申请
    DNA SEQUENCING METHOD AND SYSTEM 有权
    DNA序列方法和系统

    公开(公告)号：US20110045992A1

    公开(公告)日：2011-02-24

    申请号：US12742995

    申请日：2008-11-17

    申请人： Zhifeng Shao ,  Sitong Sheng

    发明人： Zhifeng Shao ,  Sitong Sheng

    IPC分类号： C40B30/00 ,  C12Q1/68 ,  C12M1/34 ,  C40B60/12

    CPC分类号： C12Q1/6874 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/501 ,  C12Q2565/531

    摘要： The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.

    摘要翻译： 本发明涉及基因工程领域，提供了DNA测序方法和系统。 所述DNA测序方法包括以下步骤：A.将所述DNA加工成多个DNA片段，然后构建成多个DNA标签; B.扩增每个单个DNA标签，然后加工成单链DNA; C.利用可连接DNA标签的锚，并具有至少一个退化碱基来对每一个DNA标签进行排序，从而产生测序信号; D.通过测序信号获得每个DNA片段的序列。 所述DNA测序系统包括：短标签构建单位，放大单位，测序反应单位和信号处理单位。 在本发明中，由于通过使用具有至少一个退化性的测序锚来对DNA标签进行测序，所以扩展了可直接测序的DNA标签的长度。 短和长DNA标签都可以测序。 因此扩展了DNA测序的应用。

    FLUID CELL AND GENE SEQUENCING REACTION PLATFORM AND GENE SEQUENCING SYSTEM
    5.
    发明申请
    FLUID CELL AND GENE SEQUENCING REACTION PLATFORM AND GENE SEQUENCING SYSTEM 审中-公开
    流体细胞和基因序列反应平台和基因测序系统

    公开(公告)号：US20110124094A1

    公开(公告)日：2011-05-26

    申请号：US13054249

    申请日：2009-07-16

    申请人： Sitong Sheng

    发明人： Sitong Sheng

    IPC分类号： C12M1/40

    CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  C12Q1/6874

    摘要： In the invention, a fluid cell, used for sequencing reaction between DNA fragments and reagents, comprises: a reaction chamber, one inner side of which is fixed with multiple DNA fragments; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. Multiple DNA fragments are fixed on the reaction chamber with the small capacity as short tag arrays in sequence and make the reaction go on at the condition of the reagents without diffusion barriers, which improves the contacts of reagents and DNA fragments so as to shorten the reaction time. At the same time, the demands to the concentration and dosage of reagents are low, which reduces the consumption of reagents. As the result, the sequencing cost cut down. Multiple DNA fragments are fixed on the fluid cell at the same time, which provides a way for parallel reaction of various fragments. The automatic high-through sequencing and fast sequencing reactions are achieved in the invention.

    摘要翻译： 在本发明中，用于对DNA片段和试剂之间的反应进行测序的流体细胞包括：反应室，其内侧固定有多个DNA片段; 试剂入口和试剂出口，其分别位于反应室的另一内侧的每个端部，并用于反应室中流入和流出的试剂。 多个DNA片段依次以小容量作为短标签阵列固定在反应室上，使反应在没有扩散阻挡层的试剂条件下进行，这改善了试剂和DNA片段的接触以缩短反应 时间。 同时，对试剂浓度和用量的要求也很低，从而降低试剂的消耗。 因此，排序成本降低。 多个DNA片段同时固定在流体细胞上，为各种片段的平行反应提供了途径。 在本发明中实现了自动高通量测序和快速测序反应。