公开(公告)号：US20220033845A1

公开(公告)日：2022-02-03

申请号：US17277663

申请日：2019-09-23

申请人： Zhimei DU ,  Bo JIANG ,  Xiaoyan TANG ,  Merck Sharp & Dohme Corp.

发明人： Zhimei Du ,  Bo Jiang ,  Xiaoyan Tang

IPC分类号： C12N15/85 ,  C07K16/06

摘要： The invention provides expression vectors for expressing recombinant proteins (e.g., biologics) in mammalian cells. Also provided are host cells comprising the expression vectors, methods of producing the recombinant proteins, and methods of propagating the expression vectors.

公开(公告)号：US20220089775A1

公开(公告)日：2022-03-24

申请号：US17536716

申请日：2021-11-29

申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  MERCK SHARP & DOHME CORP

发明人： Lawrence Chasin ,  Qinghao Zhang ,  Zhimei Du

IPC分类号： C07K16/32 ,  C12N15/87 ,  C12N15/64

摘要： The present invention provides, inter alia, a multiply auxotrophic cell line that is deficient in genes encoding enzymes that catalyze steps in the de novo synthesis of the pyrimidine and purine pathways, such as, e.g., uridine monophosphate synthetase (UMPS) and 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), respectively, for the production of recombinant proteins such as recombinant monoclonal and bispecific antibodies. Methods for preparing the multiply auxotrophic, in particular the doubly auxotrophic and octa-auxotrophic cell lines disclosed herein, methods for selecting a cell expressing a protein of interest, methods for producing a protein of interest, methods for optimizing the activity of a protein of interest, and kits for selecting a cell expressing a protein of interest, are also provided. In addition, recombinant proteins such as antibodies, including monoclonal and bispecific antibodies, made by the methods of the present disclosure are also provided.

公开(公告)号：US20220073945A1

公开(公告)日：2022-03-10

申请号：US17416286

申请日：2019-12-20

申请人： Merck Sharp & Dohme Corp.

发明人： Zhimei Du ,  Balrina Gupta ,  Steven C. Huhn

IPC分类号： C12N15/85 ,  C12N15/11 ,  C12N15/67

摘要： The invention provides expression vectors for expressing multi-chain recombinant proteins (e.g., biologics) in mammalian cells. Also provided are host cells comprising the expression vectors, methods of producing the multi-chain recombinant proteins, and methods of propagating the expression vectors.