利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

82 条记录 (耗时 0.035 秒)

    Method for characterization of modifications caused by the use of designer nucleases
    6.
    发明授权
    Method for characterization of modifications caused by the use of designer nucleases 有权

    公开(公告)号：US11319580B2

    公开(公告)日：2022-05-03

    申请号：US17277236

    申请日：2019-09-19

    申请人： Albert-Ludwigs-Universitaet Freiburg ,  Deutsches Krebsforschungszentrum Stiftung des oeffentlichen Rechts

    发明人： Toni Cathomen ,  Giandomenico Turchiano ,  Georges Blattner ,  Gianni Monaco ,  Melanie Boerries ,  Geoffroy Andrieux

    IPC分类号： C12Q1/6848 ,  C12Q1/6869

    摘要： Disclosed is a method for high-throughput detection of genome-wide modifications in a nucleic acid genome obtained from a cell or tissue caused by the activity of a designer nuclease comprising the following steps: a) Extraction of the genomic DNA from cells that were exposed to a designer nuclease under conditions which allow the designer nuclease to introduce a DNA double-strand break (DSB) in the genomic DNA of the cell, b) fragmentation of the nucleic acid to obtain random fragments, c) performing an end repair in order to obtain blunt ends, d) ligation with a linker comprising a sequence complementary to a so called “linker primer”, e) performing a first nucleic acid amplification reaction with a “linker primer” and a so called “ON-target primer”, whereby one primer is located upstream and one primer is located downstream of the on-target site, wherein at least one decoy primer is present in the reaction mixture, f) performing a second nucleic acid amplification reaction whereby so called “nested primers” are added to the reaction mixture, whereby one primer is complementary to the on-target locus and one primer complementary to the linker sequence, g) performing a further nucleic acid amplification reaction whereby at least one code containing primers are added to the reaction mixture, h) sequencing of the nested and barcoded amplification product, and i) aligning the sequenced products with suitable bioinformatic means to a reference sequence to identify a chromosomal location that contains a genomic modification based on at least one DNA double strand break.

    METHOD AND DEVICE FOR ASCERTAINING A NETWORK CONFIGURATION OF A NEURAL NETWORK
    8.
    发明申请
    METHOD AND DEVICE FOR ASCERTAINING A NETWORK CONFIGURATION OF A NEURAL NETWORK 有权

    公开(公告)号：US20210012183A1

    公开(公告)日：2021-01-14

    申请号：US16978108

    申请日：2019-04-17

    申请人： Robert Bosch GmbH ,  Albert-Ludwigs-Universitaet Freiburg

    发明人： Thomas Elsken ,  Frank Hutter ,  Jan Hendrik Metzen

    IPC分类号： G06N3/063 ,  G06K9/62 ,  G06N3/08 ,  G06N7/00

    摘要： A method for ascertaining a suitable network configuration for a neural network. The method includes: a) providing an instantaneous network configuration set that includes network configurations corresponding to a Pareto set with regard to a prediction error and at least one further optimization target; b) providing a set of network configuration variants; c) selecting network configurations from the set of network configuration variants based on a probability distribution of the network configurations of the instantaneous network configuration set with regard to the at least one further optimization target; d) training neural networks of each of the selected network configurations and determining a corresponding prediction error; e) updating the instantaneous network configuration set as a function of the prediction errors and the at least one further optimization target of the network configuration set and the selected network configurations; and f) selecting the suitable network configuration from the instantaneous network configuration set.

    Fluidic module, device and method for aliquoting a liquid
    9.
    发明授权
    Fluidic module, device and method for aliquoting a liquid 有权

    公开(公告)号：US10882039B2

    公开(公告)日：2021-01-05

    申请号：US15089317

    申请日：2016-04-01

    申请人： Hahn-Schickard-Gesellschaft fuer angewandte Forschung e.V. ,  Albert-Ludwigs-Universitaet Freiburg

    发明人： Frank Schwemmer ,  Steffen Zehnle ,  Nils Paust ,  Pierre Dominique Kosse ,  Daniel Mark

    IPC分类号： B01L3/00

    摘要： A fluidic module includes first and second measuring chambers, first and second fluid inlet channels connected to the first and second measuring chambers, respectively, and first and second fluid outlet channels connected to the first and second measuring chambers, respectively. Upon rotation of the fluidic module about a center of rotation, liquids are centrifugally driven into the first and second measuring chambers via the first and second fluid inlet channels, respectively, so that compressible media previously present within the first and second measuring chambers are compressed by the liquids driven into the first and second measuring chambers, respectively. Upon a reduction of the rotational frequency and upon an expansion, resulting therefrom, of the compressible media, the liquids present within the first and second measuring chambers are driven out of same via the first and second fluid outlet channels, respectively.