公开(公告)号：US11865508B2

公开(公告)日：2024-01-09

申请号：US17008425

申请日：2020-08-31

申请人： Arizona Board of Regents on behalf of Arizona State University

发明人： Neal Woodbury ,  Zhang-Gong Zhao

IPC分类号： B01J19/00 ,  C07K1/04 ,  C40B50/18 ,  C40B80/00

CPC分类号： B01J19/0046 ,  C07K1/047 ,  C40B50/18 ,  C40B80/00 ,  B01J2219/00432 ,  B01J2219/00596 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00725

摘要： Described herein are in situ synthesized arrays and methods of making the them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

公开(公告)号：US11654411B2

公开(公告)日：2023-05-23

申请号：US17205263

申请日：2021-03-18

申请人： Illumina Cambridge Limited

发明人： Mark Edward Brennan Smith ,  Andrea Sabot ,  Isabelle Marie Julia Rasolonjatovo ,  Jean-Ernest Sohna Sohna ,  Adrian Martin Horgan ,  Harold Philip Swerdlow

IPC分类号： B01J19/00 ,  C40B50/18 ,  C12Q1/6834 ,  C40B40/06 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6837 ,  C08F222/38

CPC分类号： B01J19/0046 ,  C08F222/38 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/18 ,  B01J2219/0061 ,  B01J2219/0072 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00716 ,  B01J2219/00722 ,  Y10T428/265

摘要： Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

公开(公告)号：US20180149611A1

公开(公告)日：2018-05-31

申请号：US15823610

申请日：2017-11-28

申请人： Caduceus Biotechnology Inc. ,  HELIOS BIOELECTRONICS INC.

发明人： Golden Tiao ,  Rung-Ywan Tsai ,  Wen-Yih Chen

IPC分类号： G01N27/327 ,  G01N27/406 ,  G01N27/417

CPC分类号： G01N27/3275 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00653 ,  G01N27/406 ,  G01N27/4145 ,  G01N27/417 ,  G01N33/54353 ,  G01N33/54373

摘要： A biosensors and a method of forming a probe on a solid surface of a biosensor are disclosed and the method includes the following steps. A unit (a nucleotide or an amino acid) capped with a protecting group is covalently bonded on the solid surface of one of a plurality of sensor cells of the biosensor. At least one cycle of the following steps is performed until a desired number of units is formed: irradiating the one of the plurality of sensor cells, so as to remove the protecting group of the unit; and binding a unit capped with the protecting group to the de-protected unit. The one of the plurality of sensor cells is irradiated to remove the protecting group.

公开(公告)号：US20180104663A1

公开(公告)日：2018-04-19

申请号：US15622661

申请日：2017-06-14

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Alexei Finski ,  Gavin MacBeath

IPC分类号： B01J19/00 ,  G01N33/50 ,  G01N33/68

CPC分类号： B01J19/0046 ,  B01J2219/00596 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00702 ,  B01J2219/00725 ,  G01N33/5091 ,  G01N33/6803 ,  G01N33/6848

摘要： The invention provides a method for the isolation of a single cell embedded in a tissue while preserving the state of molecules of the cell, and therefore allows for transformation of a single target cell in live tissue into a format that can be evaluated using analytical methods.

公开(公告)号：US20170369936A1

公开(公告)日：2017-12-28

申请号：US15425882

申请日：2017-02-06

申请人： Kerry B. Gunning ,  Mark Aaron Behlke

发明人： Kerry B. Gunning ,  Mark Aaron Behlke

IPC分类号： C12Q1/68 ,  C12P19/34 ,  B01J19/00

CPC分类号： C12Q1/6837 ,  B01J19/0046 ,  B01J2219/0059 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6806 ,  Y10T436/108331 ,  C12Q2525/125 ,  C12Q2533/101 ,  C12Q2521/319

摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.

公开(公告)号：US20170354967A1

公开(公告)日：2017-12-14

申请号：US15679111

申请日：2017-08-16

申请人： CapitalBio Corporation ,  Tsinghua University

发明人： Shengce TAO ,  Jing CHENG ,  Xuemei MA ,  Qiong ZHANG ,  Yuxiang ZHOU

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C40B40/06 ,  B01L7/00 ,  C40B60/14

CPC分类号： B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

摘要： This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

公开(公告)号：US20170240886A1

公开(公告)日：2017-08-24

申请号：US15440293

申请日：2017-02-23

申请人： GINKGO BIOWORKS, INC.

发明人： Andrew V. Oleinikov

IPC分类号： C12N15/10

CPC分类号： C12N15/1068 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00432 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B50/14 ,  C40B80/00

摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

公开(公告)号：US09675954B2

公开(公告)日：2017-06-13

申请号：US14402693

申请日：2013-05-23

申请人： ALBERT-LUDWIGS-UNIVERSITAET FREIBURG

发明人： Guenter Roth

IPC分类号： B01J19/00 ,  C40B20/02

CPC分类号： B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01J2219/00725

摘要： The invention relates to a method for producing a microarray, wherein the production of this array is detected in real time from the accumulation of the product molecules being produced. The invention further relates to a microarray produced by this method, and to a device for the real-time detection of molecular accumulations on an array surface during the production of microarrays.

公开(公告)号：US09623394B2

公开(公告)日：2017-04-18

申请号：US13983449

申请日：2012-02-03

申请人： Günter Roth ,  Jürgen Burger

发明人： Günter Roth ,  Jürgen Burger

IPC分类号： B01J19/00 ,  B01L3/00 ,  B82Y30/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00337 ,  B01J2219/00495 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/508 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  B82Y30/00

摘要： The invention relates to a device and a method for the generation of molecular microarrays. The invention relates therefore to a universal approach for the generation of protein microarrays, DNA microarrays and RNA microarrays (in general nucleic acid microarrays), by production of an output molecule from a template molecule microarray via enzymatic or chemical processes and transfer of the output molecule onto the desired molecular microarray.

公开(公告)号：US09599611B2

公开(公告)日：2017-03-21

申请号：US11912565

申请日：2006-04-25

申请人： M. Selim Unlu ,  David A. Bergstein ,  Michael F. Ruane ,  Bennett B. Goldberg

发明人： M. Selim Unlu ,  David A. Bergstein ,  Michael F. Ruane ,  Bennett B. Goldberg

IPC分类号： C40B30/04 ,  G01N33/543 ,  C12Q1/68 ,  G01N21/45 ,  G01N21/75 ,  G02B21/34 ,  C40B40/06 ,  G01N21/77

CPC分类号： G01N21/77 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/45 ,  G01N21/75 ,  G01N33/54373 ,  G01N2021/7779 ,  G01N2201/061 ,  G02B21/34 ,  C12Q2565/619

摘要： This disclosure provides methods and devices for the label-free detection of target molecules of interest. The principles of the disclosure are particularly applicable to the detection of biological molecules (e.g., DNA, RNA, and protein) using standard SiO2-based microarray technology.