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公开(公告)号：US09599629B2
公开(公告)日：2017-03-21
申请号：US14541708
申请日：2014-11-14
Applicant: Cytoskeleton Inc.
Inventor： Ashley Middleton-Davis , Kim Middleton-Davis
CPC classification number: G01N35/1002 , B01L3/52 , B01L9/52 , B01L99/00 , B01L2200/141 , B01L2200/16 , B01L2300/023 , B01L2400/0457 , B01L2400/0487 , G01N33/5302 , G01N33/54366 , G01N35/00 , G01N35/00722 , G01N35/0092 , G01N2035/00277
Abstract: The present disclosure provides devices which deliver fluids from several reservoirs to a reaction vessel and eventually to a waste chamber in a predetermined schedule. The device provides improved simplicity while improving operational robustness and flexibility.
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公开(公告)号：US20100240551A1
公开(公告)日：2010-09-23
申请号：US12792130
申请日：2010-06-02
Applicant: Li Cheng , Ashley Davis , Kim Middleton
Inventor： Li Cheng , Ashley Davis , Kim Middleton
IPC: G01N33/573 , C40B30/04
CPC classification number: G01N33/573 , G01N2333/916
Abstract: The present invention provides methods of detecting activated Rho GTPase proteins by contacting a solid support with a sample comprising an activated Rho GTPase protein. The solid support is linked to an activated Rho GTPase binding peptide. The activated Rho GTPase protein remains associated with the solid support during the detection.
Abstract translation: 本发明提供了通过使固体支持物与包含活化的Rho GTP酶蛋白质的样品接触来检测活化的Rho GTP酶蛋白质的方法。 固体支持物与活化的Rho GTP酶结合肽连接。 活化的Rho GTPase蛋白在检测期间与固体支持物相关。
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Patent Agency Ranking
公开(公告)号：US07282571B2
公开(公告)日：2007-10-16
申请号：US10771595
申请日：2004-02-03
Applicant: Ashley Stuart Davis , Kim Maria Middleton
Inventor： Ashley Stuart Davis , Kim Maria Middleton
IPC: C07K14/435
CPC classification number: C07K14/4716
Abstract: In basic research and drug discovery there is a need to provide protein reagents with high activity. In particular proteins that are used in assays must have a good shelf life and a consistent activity. As a system of measuring actin polymerization, pyrene labeled actin was developed in 1983 by Kouyama and Mihashi. However, prior to this disclosure, pyrene actin has been impossible to store for longer than one month because the activity would be lost due to protein denaturation and frozen pyrene actin immediately lost activity. This disclosure relates a method for stabilizing pyrene labeled actin for extended storage times and an optimal storage buffer thus creating a reproducible source of pyrene actin for various assays. Using the processes described herein, pyrene actin has been stored for greater than 3 years at 4° C. which makes it a viable product for retail.
Abstract translation: 在基础研究和药物发现方面，需要提供高活性的蛋白质试剂。 特别是用于测定中的蛋白质必须具有良好的保质期和一致的活性。 作为测量肌动蛋白聚合的系统，1983年由Kouyama和Mihashi开发了芘标记的肌动蛋白。 然而，在本公开之前，芘肌动蛋白不可能存储超过一个月，因为活性将由于蛋白质变性而丧失，并且冷冻的芘肌动蛋白立即失去活性。 本公开涉及一种用于稳定芘标记的肌动蛋白以延长储存时间的方法和最佳储存缓冲液，从而为各种测定产生芘肌动蛋白的可重复来源。 使用本文所述的方法，芘肌动蛋白已经在4℃下储存超过3年，这使其成为零售的可行产品。
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公开(公告)号：US07259278B2
公开(公告)日：2007-08-21
申请号：US09725030
申请日：2000-11-29
Applicant: Ashley Stuart Davis , Kim Maria Middleton , Jain Dong Jiang , J. George Bekesi
Inventor： Ashley Stuart Davis , Kim Maria Middleton , Jain Dong Jiang , J. George Bekesi
IPC: C07C237/28
CPC classification number: C07C233/54 , A61K45/06
Abstract: It is presently accepted that the mechanism of action for all anti-tumor tubulin ligands involves the perturbation of microtubule dynamics during the G2/M phase of cell division and subsequent entry into apoptosis (1). In this invention, we report a novel tubulin ligands which have a unique mechanism of action. These compounds, halogenated derivatives of acetamido benzoyl ethyl ester (HAABE), arrest cancer cells in S-phase and cause cell death by a combination of apoptosis (DNA ladder) and necrosis (DNA degradation) type mechanisms. Normal cells are not affected at the same concentrations of compound. The ligands bind covalently to tubulin in vitro and in vivo and shows potent cancericidal activity in tissue culture assays and in animal tumor models. These compounds target early S-phase at the G1/S transition rather than the G2/M phase and mitotic arrest. Bcl-2 phosphorylation, a marker of mitotic microtubule inhibition by other tubulin ligands was dramatically altered, phosphorylation was rapid and biphasic rather than a slow linear event. The halogenated ethyl ester series of derivatives thus constitute a unique set of tubulin ligands which induce a novel mechanism of cancer cell death.
Abstract translation: 目前所接受的是，所有抗肿瘤微管蛋白配体的作用机制涉及在细胞分裂的G2 / M期和随后进入凋亡期间微管动力学的扰动（1）。 在本发明中，我们报道了一种具有独特作用机制的新型微管蛋白配体。 这些化合物，乙酰氨基苯甲酰基乙酯（HAABE）的卤化衍生物通过凋亡（DNA梯度）和坏死（DNA降解）类型机制的组合阻止S期的癌细胞并引起细胞死亡。 正常细胞在相同浓度的化合物下不受影响。 配体在体外和体内共价结合到微管蛋白上，并且在组织培养测定和动物肿瘤模型中显示出有效的杀癌活性。 这些化合物在G1 / S转换期间靶向早期S期，而不是G2 / M期和有丝分裂停滞。 Bcl-2磷酸化，其他微管蛋白配体的有丝分裂微管抑制的标志物显着改变，磷酸化是快速和双相而不是缓慢的线性事件。 卤代乙酯系列衍生物因此构成了一组独特的微管蛋白配体，其诱导癌细胞死亡的新机制。
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公开(公告)号：US10690573B2
公开(公告)日：2020-06-23
申请号：US15604809
申请日：2017-05-25
Applicant: Cytoskeleton, Inc.
Inventor： Kim Middleton , Soonjin Hong , Wai K. Law , Henrick Horita
IPC: G01N1/40 , G01N1/38 , G01N33/543 , G01N33/68
Abstract: The present disclosure provides methods for reducing the viscosity of a cell lysate or tissue lysate by: contacting the cell lysate or tissue lysate with a compressible and open-cell foam filter having a pore size from about 0.65 mm to about 1.22 mm, compressing the filter to recover the lysate absorbed in the filter, and collecting the filtered lysate; and also provides kits therefor.
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公开(公告)号：US10288636B2
公开(公告)日：2019-05-14
申请号：US15427334
申请日：2017-02-08
Applicant: Cytoskeleton, Inc.
Inventor： Ashley Middleton-Davis , Kim Middleton-Davis
Abstract: The present disclosure provides devices which deliver fluids from several reservoirs to a reaction vessel and eventually to a waste chamber in a predetermined schedule. The device provides improved simplicity while improving operational robustness and flexibility.
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公开(公告)号：US07763418B2
公开(公告)日：2010-07-27
申请号：US11428415
申请日：2006-07-03
Applicant: Li Cheng , Ashley Davis , Kim Middleton
Inventor： Li Cheng , Ashley Davis , Kim Middleton
IPC: C12Q1/00 , G01N33/53 , G01N33/566
CPC classification number: G01N33/573 , G01N2333/916
Abstract: The present invention provides methods of detecting activated Rho GTPase proteins by contacting a solid support with a sample comprising an activated Rho GTPase protein. The solid support is linked to an activated Rho GTPase binding peptide. The activated Rho GTPase protein remains associated with the solid support during the detection.
Abstract translation: 本发明提供了通过使固体支持物与包含活化的Rho GTP酶蛋白质的样品接触来检测活化的Rho GTP酶蛋白质的方法。 固体支持物与活化的Rho GTP酶结合肽连接。 活化的Rho GTPase蛋白在检测期间与固体支持物相关。
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